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鳗弧菌(Vibrio anguillarum)对青蛤(Cyclina sinensis)的毒性及半致死浓度研究
葛端阳, 赵 婷, 潘宝平
天津师范大学生命科学学院 天津市动植物抗性重点实验室
摘要:
利用不同浓度的鳗弧菌对青蛤进行了急性毒性实验, 观察青蛤在菌液胁迫下的存活情况, 进一步统计得到鳗弧菌对青蛤的半致死浓度。结果表明, 青蛤在鳗弧菌液浓度OD600为1.0—2.2范围内, 胁迫96h以内死亡率呈逐渐上升趋势, 并且在OD600= 2.2时所有受试个体全部死亡。在相同的胁迫时间内, 受试个体死亡率与胁迫浓度均呈正相关。经SPSS16.0软件分析后, 最终得到鳗弧菌对青蛤的半致死浓度(LC50)为OD600= 1.57, 说明鳗弧菌对青蛤有明显的毒害作用。
关键词:  青蛤, 鳗弧菌, 半致死浓度
DOI：10.11693/hyhz201206023023
分类号:
基金项目:天津市科委应用基础与前沿技术重点项目资助, 12JCZDJC22800 号, 09JCZDJC19300 号。
TOXICITY TEST AND HALF LETHAL CONCENTRATION OF VIBRIO ANGUILLARUM TO THE CLAM CYCLINA SINENSIS
GE Duan-Yang, ZHAO Ting, PAN Bao-Ping
College of Life Sciences, Tianjin Key Laboratory of Animal and Plant Resistance, Tianjin Normal University
Abstract:
The acute toxicity test was conducted under different concentration of Vibrio anguillarum to Cyclina sinensis. The death rate of the C. sinensis were observed, then the half lethal concentration of V. anguillarum was got after infection. The results showed that the death rate of the clam was increased at the Vibrio concentration from OD600= 1.0 to OD600= 2.2 in 96h, and all the experimental individuals were dead at the concentration OD600= 2.2. The death rate and the Vibrio concentration showed a positive correlation at the same time point. After analyzing the experimental data by SPSS 16 package, we got that the LC50 of Vibrio anguillarum to C. sinensis was OD600= 1.57. The results suggested that the V. anguillarum was obviously toxic to C. sinensis.
Key words:  Cyclina sinensis, Vibrio anguillarum, Semi-lethal concentration (LC50)
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