| 摘要: |
| 采用中心组合设计和响应曲面法在实验室条件下研究了Cd2+与Zn2+对马氏珠母贝受精率的影响。Cd2+、Zn2+的浓度范围分别为0.030—20mg/L、0.100—20mg/L。结果表明: Cd2+与Zn2+两因子对马氏珠母贝受精率的一次效应极显著( P <0.01); Cd2+与Zn2+两因子一次互作效应极显著( P <0.01) 。经响应曲面法分析, 随着Cd2+与Zn2+浓度的增加受精率呈下降趋势, 当Zn2+浓度在最低浓度(0.100mg/L)时, 受精率随着Cd2+浓度的上升而下降; 当Cd2+浓度在最低浓度(0.030mg/L)时, 受精率随Zn2+浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd2+、Zn2+间关系的模型方程( R2 = 0.996, Adj.R2 = 0.987, Pred.R2 = 0.887, P <0.01) 并可用于预测Cd2+、Zn2+对马氏珠母贝受精率的影响。 |
| 关键词: 马氏珠母贝, 受精率, Cd2+, Zn2+ |
| DOI：10.11693/hyhz201202020020 |
| 分类号: |
| 基金项目:广东省教育厅工程中心建设项目, GCZX-A0909 号; 广东省科技厅科技计划重大专项, 2010B020201014 号; 科技部国家星火计划项目, 2011GA780001 号 |
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| COMBINED EFFECTS OF Cd2+ AND Zn2+ ON THE FERTILIZATION RATE IN PINCTADA MARTENSII (DUNKER) |
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DENG Yun-Cui, WANG Hui, LI Yan-Hong, ZHU Xiao-Wen, LUO Ming-Ming, WANG Ya-Nan
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Fisheries College of Guangdong Ocean University
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| Abstract: |
| Using the central composite face-centered design (CCF) and response surface methodology, the combined effects of Cd2+ (0.030—20.000mg/L) and Zn2+ (0.100—20.000mg/L) on the fertilization rate in Pinctada martensii were studied under laboratory conditions. The results showed that the effects of two factors Cd2+ and Zn2+ on the fertilization rate in P. m a r t e n s i i were highly significant ( P <0.01), and effect of Cd2+ was more significant than that of Zn2+. The inter-action between the two factors was also highly significant (P <0.01). Based on the response surface plots, it was found that with the increased concentration of Cd2+ and Zn2+ , the fertilization rate was decreased. When Zn2+ concentration was at its lowest level (0.100mg/L), the fertilization rate decreased with the increased Cd2+ concentration. When Cd2+ concentration was at its lowest level (0.030mg/L), the fertilization rate was first decreased and then increased as the concentration of Zn2+ increased. The model equation of the fertilization rate towards Cd2+ and Zn2+ was established, with R2 = 0.996, Adj.R2 = 0.987 and Pred. R2 = 0.887 (P <0.01). The model equation could be practically used for forecasting the effects of Cd2+ and Zn2+ on the fertilization rate in P. martensii. |
| Key words: Pinctada martensii (Dunker), Fertilization rate, Cd2+, Zn2+ |
