| 摘要: |
| 采用ISSR分子标记对中国沿海4个厚壳贻贝群体进行遗传多样性和遗传结构分析, 筛选出13 条引物对 4 个群体 120 个样品共扩增出 192 个清晰的扩增位点, 其中多态位点 188 个, 多态位点百分率为 97.92%, 在物种水平上遗传多样性较为丰富。群体遗传多样性结果表明, PPL 在 81.77%—89.58%之间, H在 0.2950—0.2818之间, I在 0.4213—0.4447之间, 其中福州群体多样性指数最低, 温州群体最高。4 个群体间 GST 为 0.1519, Nm 值范围介于 3.0576—5.9714 之间, AMOVA 分析显示遗传变异主要来自群体内个体间。群体间遗传距离和聚类分析显示, 温州群体和宁德群体首先聚为一支, 再与舟山群体聚类, 最后与福州群体聚在一起。表明地理位置较远的群体, 遗传距离也较大, 地理距离和遗传距离一致。 |
| 关键词: 厚壳贻贝, 群体遗传, 遗传多样性, ISSR |
| DOI：10.11693/hyhz201201019019 |
| 分类号: |
| 基金项目:国家科技部国际科技合作项目“浅海典型渔业生态系统功能恢复与重建关键技术”, 2009DFB20290 号; 国家海洋公益性行业科研专项, 201005013-05 号; 农业部海洋与河口渔业资源及生态重点开放实验室开放基金, 10-01 号; 2011 年浙江省大学生科技创新(新苗人才计划)项目, 2011.01—2012.06。 |
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| ISSR ANALYSIS ON GENETIC STRUCTURE OF FOUR MYTILUS CORUSCUS POPULATIONS |
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YE Ying-Ying1, XU Mei-Ying1,2, GUO Bao-Ying1,3, WU Chang-Wen1
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1.Marine Science College of Zhejiang Ocean University, National Engineering Research Center of Marine Facilities Aquaculture;2.Zhejiang Dahaiyang Science and Technology Co., Ltd.;3.Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology, Ministry of Agriculture, East China Sea Fisheries R
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| Abstract: |
| The genetic structure of four geographic populations of Mytilus coruscus along the coast of China was investigated by the inter-simple sequence repeat (ISSR) fingerprinting of 120 individual clams. Of the 192 ISSR loci tested, 188 (97.92%) were polymorphic with 13 ISSR primers. The genetic diversity was high at species level. The result showed that the PPL about four populations were among 81.77%—89.58%, H were among 0.2950—0.2818, I were among 0.4213—0.4447, respectively for the four populations with the lowest values in FZ population, the highest values in WZ population. The GST of the four populations was 0.1519, Nm was 3.0576—5.9714, AMOVA analysis showed that the genetic variation mainly from individuals within populations. Genetic distance and UPGMA tree showed that WZ was clustered with ND into one clad, then with ZS, and finally with FZ. The result showed that the further geographical distance, the higher genetic distance; the genetic distance is proportional to the geographical distance. |
| Key words: Mytilus coruscus, Population genetics, Genetic diversity, ISSR |
