| 摘要: |
| 采用SSR方法进行了闽粤群(MY)和岱衢群(DQ)及其正交和反交大黄鱼的遗传差异的研究。结果表明, Shannon’s 多样性指数为 DQ 群高于 MY 群, 分别为 2.14、2.04; 反交群高于正交群分别为2.13、2.03; 杂交群体中为 2.24 与其亲体群的相似(2.22)。亲体群间基因分化系数 Fst 值为 0.020, 低于杂交后代的 0.026。AMOVA 显示, 亲体群和后代群基因差异均很低, 且亲体群基因分化度低于和杂交后代群, Fst 值分别为 0.011、0.032。Nei 遗传距离显示子代间遗传距离大与亲代代间的遗传距离分别为 0.39、0.22。UPGMA 聚类将其分为 3 组, 其中 DQ 群与 MY 群遗传距离最近。可以认为养殖的DQ群与MY群大黄鱼间遗传背景差异较低, 其中DQ族遗传背景较好, 杂交后代群的基因多样性和基因差异性稍有提高, 其中反交效果较好。 |
| 关键词: 大黄鱼, 基因多样性, 微卫星, 正、反交 |
| DOI：10.11693/hyhz201104020020 |
| 分类号: |
| 基金项目:国际科技合作项目, 2006DFA32960 号; 国家公益性行业科研专项, 200903029-06 号 |
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| STUDY OF CULTURED DAIQU AND MINYUE POPULATIONS LAGER YELLOW CROAKER PSEUDOSCIAENA CROCEA AND ITS HYBRID FILIAL GENERATIONS USING SSR TECHNOLOGY |
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HUANG Zhen-Yuan1,2, SU Yong-Quan1, ZHANG Jian-She2, WU Chang-Wen2
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1.College of Oceanography and Environmental Science, Xiamen University;2.Marine Science College of Zhejiang Ocean University, Key Laboratory of Mariculture Equipments and Engineering Technology of Zhejiang Province
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| Abstract: |
| The genetic diversity of 120 accessions of breeding Large Yellow Croaker, Pseudosciaena crocea (Richardson) was analyzed. Using 11 pair of SSR polymorphic markers, a total of 26 alleles were found, which exhibited high level of diversity. The range of polymorphism information content (PIC) was 0.785—0.818, average 0.804. Shannon’s Information index of DQ populations was higher than MY populations and it was 2.14, 2.04 respectively. The filial generation higher than parental generation it was 2.24, 2.22 respectively. And minus-hybrid generation higher than plus-hybrid generation it was 2.13, 2.03 respectively. The Fst of parental generation was lower than finial generation; it was 0.020, 0.026 respectively. The Gene flow was very high according to Fst value. The UPGMA clustering analysis at the genetic similarity grouped the four populations into three groups. The minus-hybrid stock was the most far away from others populations. AMOVA in filial generation higher than parental generation it P value was 0.01, 0.03 respectively. |
| Key words: Large yellow croaker Pseudosciaena crocea, Genetic diversity, Microsatellites, Reciprocal crosses hybrid |
