| 摘要: |
| 以致病性溶藻弧菌为指示菌, 通过酸粗提、固相萃取、Sephadex G-25 柱凝胶层析和RP-HPLC 等分离纯化技术, 从大黄鱼胃肠组织中分离纯化抗菌肽。结果表明, 大黄鱼肠道组织中存在着多种抗菌物质, 并最终得到一种高纯度的新型抗菌肽 AMP-W3。AMP-W3 由 10 种氨基酸组成, 其中天冬氨酸、谷氨酸和甘氨酸的含量较高。AMP-W3 对溶藻弧菌的最低抑菌浓度介于 1.25—2.5?g/ml, 对人血红细胞的溶血浓度为 1.25?g/ml, 在酸性条件下的抗菌活性更强, 对革兰氏 |
| 关键词: 抗菌肽, 纯化, 生物活性, 大黄鱼 |
| DOI：10.11693/hyhz201103018018 |
| 分类号: |
| 基金项目:国家“863”高技术研究发展计划, 2007AA09Z115 号; 农业部公益性行业科研专项, 200903029 号 |
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| ISOLATION AND CHARACTERIZATION OF A NOVEL ANTIMICROBIAL PEPTIDE FROM THE GASTROINTESTINAL TRACT OF LARGE YELLOW CROAKER PSEUDOSCIAENA CROCEA |
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JI Yong1, WANG Xiao-Lu1, SU Yong-Quan2, QIN Ying-Xue1, ZOU Wen-Zheng1, XING Yan-Li1, XU Xiao-Jin1, YAN Qing-Pi1
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1.Fisheries College of Jimei University, Key Laboratory of Science and Technology for Aquaculture and Food Safety;2.Department of Oceanography, Xiamen University
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| Abstract: |
| The purpose of this study was to obtain antimicrobial peptide from the gastrointestinal tract of Pseudosciaena crocea. Our results showed that a novel antimicrobial peptide to Vibrio alginolyticus was isolated and purified using acid extraction, solid phase extraction, Sephadex G25 and reverse phase high performance liquid chromatography. The antimicrobial peptide, which was named AMP-W3, contained 10 different amino acids and was enriched in Glu, Gly and Asp. Minimal inhibition concentrations of the peptide were at 1.25—2.5 g/ml. Moreover, AMP-W3 were hemolytic for human red blood cells when its concentration was higher than 1.25?g/ml. In the acid condition, this peptide showed higher antimicrobial activity than in the alkaline solution, and displayed a broad spectrum of antimicrobial activity against Gram-negative, Gram-positive bacteria and fungi, including Escherichia coli, Staphylococcus aureus and Aspergillus niger, etc. This is the first time that an antimicrobial peptide was isolated from the gastrointestinal tract of fish, and it may help us to understand antimicrobial peptides and the innate anti-infective defense system of fish. |
| Key words: Antimicrobial peptide, Purification, Biological activity, Pseudosciaena crocea |
