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| 6种鳗鲡(Anguilla)线粒体DNA COⅡ基因序列分析及其系统发育 |
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田鑫江1,2, 吴 宁1,2, 黎中宝1,2, 林小云1,2, 张志红1,2, 陈晓姣1,2, 陈丽娜1,2
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1.集美大学水产学院;2.集美大学水产生物技术研究所
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| 摘要: |
| 采用基因测序的方法, 对 6 种鳗鲡的 COⅡ基因片段进行了 PCR 扩增和测序。结果表明, 6种鳗鲡 COⅡ基因片段的长度都为 412bp, 4 种碱基组成非常相似, 并且 A+T 的含量都大于 G+C 的含量。 6 种鳗鲡 COⅡ基因片段核苷酸序列之间有 46bp 的差异, 其中有 6 处发生碱基颠换, 40 处发生碱基转换。 非洲鳗鲡(Anguilla mossambic)和日本鳗鲡(A. japonica)COⅡ基因片段核苷酸序列差异最大为27bp, 同源性为 93.45%, 而日本鳗鲡和菲律宾鳗鲡(A. mormorata)COⅡ基因片段核苷酸序列差异最小为 5bp, 同源性为 98.79%。 |
| 关键词: 鳗鲡, mtDNA COⅡ基因, 序列差异, 颠换, 转换 |
| DOI：10.11693/hyhz201102018018 |
| 分类号: |
| 基金项目:公益性行业(农业)科研专项经费项目, “鳗鲡种质鉴定与新品种引进”, nyhyzx07-043-08 号; 集美大学创新团队建设项目, 集大人(2007)17 号 |
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| SEQUENCING ANALYSIS OF THE mtDNA COⅡGENE FRAGMENTS OF SIX ANGUILLA SPECIES |
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TIAN Xin-Jiang1,2, WU Ning1,2, LI Zhong-Bao1,2, LIN Xiao-Yun1,2, ZHANG Zhi-Hong1,2, CHEN Xiao-Jiao1,2, CHEN Li-Na1,2
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1.Fisheries College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University
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| Abstract: |
| MtDNA COⅡgene fragments of six Anguilla species were amplified using the PCR method, and the prod-ucts were purified and sequenced. The results showed that all the mtDNA COⅡgene fragments had 412bp with similar base composition, which had greater amount of the A+T pairs than the G+C pairs. The difference among the six mtDNA COⅡgenes was 46bp, including 6 base transversions and 40 base transitions. The difference between the mtDNA COⅡ
genes of A. mossambica and A. japonica was 27bp with 93.45% homology; however the difference between those of A. japonica and A. mormorata was 5bp with 98.79% homology. |
| Key words: Anguilla, mtDNA COⅡgene, Sequence difference, Transversion, Transition |