| 摘要: |
| 利用已构建的浙江枝吻纽虫cDNA文库, 通过PCR技术扩增得到gelsolin和actin基因的全长 cDNA 序列。其中, gelsolin 的 cDNA 全长 1947bp, 5′-非翻译区 62bp, 3′-非翻译区 778bp, 开放阅读框 1107bp, 编码 369 个氨基酸; actin 的 cDNA 全长为 1830bp, 5′-非翻译区 167bp, 3′-非翻译区 532bp以及 1131bp 的开放阅读框, 编码 377 个氨基酸。序列分析表明: gelsolin 包含 3 个 gelsolin-like (G)结构域, 分别含有 Ca2+、actin、PIP2 结合位点, actin 序列中包含了 gelsolin、ATP、profiling 结合位点。比对结果表明, actin 基因氨基酸序列与其它物种的同源性达到 94%—99%。 |
| 关键词: 浙江枝吻纽虫, 凝溶胶蛋白, 肌动蛋白, 基因克隆 |
| DOI：10.11693/hyhz201102005005 |
| 分类号: |
| 基金项目:浙江省重大科技攻关项目, 2006C13089号; 浙江省教育厅科研项目, 20070951号 |
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| CLONING AND SEQUENCE ANALYSIS OF GELSOLIN AND ACTIN OF DENDRORHNCHUS ZHEJIANGENSIS |
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CHEN Lei1, LI Ye1, SU Xiu-Rong1, JIANG Jin-Po2, LI Tai-Wu1,2, LI Zhen1, ZHOU Jun1, LIU Yan1, XIA Chang-Ge1
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1.Faculty of Life Science and Biotechnology, Ningbo University;2.Ningbo City College of Vocational Technology
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| Abstract: |
| Gelsolin is a versatile actin binding protein, which can regulate actin filament remodeling in a Ca2+-dependant way. In this study, the full length cDNA of gelsolin and actin gene were cloned using the cDNA library of Dendrorhynchus zhejiangensis. The full length cDNA of gelsolin gene was 1947bp with 62bp of 5′UTR, 778bp of 3′UTR, and 1107bp of ORF, which encodes 369 amino acids. The full length cDNA of actin gene was 1830bp with 167bp of 5′UTR, 532bp of 3′UTR, and 1131bp of ORF, which encodes 377 amino acids. The analysis of these sequences showed gelsolin gene included three gelsolin-like (G) domains which contained binding sites of Ca2+, actin, and PIP2, respectively. Actin included binding sites of gelsolin, ATP, and profiling. The amino acids sequence of actin reached 94%—99% in similarity when aligned with actin proteins of other species. In addition, this study built the foundation for further research on the interaction mechanism between gelsolin and actin, and the mechanism of movement and regeneration of D. zhejiangensis. |
| Key words: Dendrorhynchus zhejiangensis, Gelsolin, Actin, Gene cloning |
