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| 6 种鳗鲡(Anguilla)线粒体 DNA COⅠ序列的比较研究 |
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吴 宁1,2, 黎中宝1,2, 林小云1,2, 张志红1,2, 田鑫江1,2, 陈晓姣1,2, 陈丽娜1,2
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1.集美大学水产学院;2.集美大学水产生物技术研究所
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| 摘要: |
| 采用基因测序的方法, 对 6 种鳗鲡的 COⅠ基因片段进行了 PCR 扩增和测序。结果表明, 6种鳗鲡 COⅠ基因片段的长度都为 652bp, 4 种碱基组成非常相似, 并且 A+T 的含量都大于 G+C 的含量。 6 种鳗鲡 COⅠ基因片段核苷酸序列之间有 105bp 的差异, 其中有 18 处发生碱基颠换, 87 处发生碱基转换。非洲鳗鲡和欧洲鳗鲡 COⅠ基因片段核苷酸序列差异最大为 54bp, 同源性为 91.72%, 而美洲鳗鲡与欧洲鳗鲡 COⅠ基因片段核苷酸序列差异最小为 24bp, 同源性为 96.32%。
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| 关键词: 鳗鲡, COⅠ基因, 序列差异, 颠换, 转换 |
| DOI：10.11693/hyhz201006020020 |
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| 基金项目:公益性行业(农业)科研专项经费项目, “鳗鲡种质鉴定与新品种引进”, nyhyzx07-043-08 号；集美大学创新团队建设项目, 2010A004 号 |
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| COMPARATIVE STUDY ON MtDNA COⅠGENE FRAGMENT OF SIX ANGUILLA |
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WU Ning1,2, LI Zhong-Bao1,2, LIN Xiao-Yun1,2, ZHANG Zhi-Hong1,2, TIAN Xin-Jiang1,2, CHEN Xiao-Jiao1,2, CHEN Li-Na1,2
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1.Fisheries College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University
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| Abstract: |
| MtDNA COⅠgene fragments of six Anguilla species were amplified by PCR, and the PCR products were purified and sequenced. The results showed that all the MtDNA COⅠgene fragments were 652bp for nucleotide sequence with similar base composition and the content of A+T were more than that of G+C. The base difference among six Anguilla species was 105bp, including 18 base transversions and 87 base transitions. The base difference between Anguilla mossambica and A. anguilla was the most in homology of 91.72%, however the base difference between A. rostrata and A. anguilla was the lost in homology of 96.32%.
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| Key words: Anguilla, COⅠgene, Sequence difference, Transversion, Transition |