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Cd2+对青蛤(Cyclina sinensis)的毒性及蓄积过程研究
张丽岩, 宋 欣, 高玮玮, 潘鹤婷, 潘宝平
天津师范大学生命科学学院 细胞遗传与分子调控天津市重点实验室
摘要:
利用水生动物急性毒性实验方法, 在6 个浓度下Cd2+对青蛤的急性毒性进行测定, 经回归分析后, 得到Cd2+对青蛤96h 半致死浓度为20.09mg/L, 安全浓度为0.201mg/L。分别对半致死浓度和安全浓度下Cd2+在青蛤体内不同组织的蓄积情况进行了分析。结果表明, 在半致死浓度条件下,168h 青蛤各组织Cd2+的含量依次为肌肉>内脏团>鳃; 而在安全浓度下, 其结果为内脏团>鳃>肌肉,内脏团对于Cd2+富集能力远高于肌肉。讨论了青蛤的养殖环境及产品检验等问题。
关键词:  青蛤, 镉, 半致死浓度, 重金属蓄积
DOI:10.11693/hyhz201003019019
分类号:
基金项目:天津市科委应用基础与前沿技术重点项目资助, 09JCZDJC19300 号; 天津市科委应用基础研究面上项目资助, 06YFJMJC11800 号
ACUTE TOXITY TEST AND ANALYSIS ON ACCUMULATION OF CADMIUM (Cd2+) TO THE CLAM CYCLINA SINENSIS
ZHANG Li-Yan, SONG Xin, GAO Wei-Wei, PAN He-Ting, PAN Bao-Ping
College of Life Sciences, Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin Normal University
Abstract:
To determine the acute toxicity Cd2+ to Cyclina sinensis, six concentrations of Cd2+ were tested. The 96-h half lethal concentration (LC50) and the safe concentration of Cd2+ for C. sinensis were 20.09mg/L and 0.201mg/L, respectively. In addition, we determined Cd2+ accumulation in C. sinensis at both the LC50 and the safe concentration. At the LC50 and 168h after the addition of Cd2+, various tissue contents of Cd2+ followed the order of muscle > visceral mass > gill; while at the safe concentration, the order was visceral mass > gill > muscle. The Cd2+ accumulating capacity of the visceral mass is much higher than that of the muscles. Our data may serve as a reference to evaluate Cd2+ distribution in C. sinensis farms and C. sinensis-based seafood products.
Key words:  Cyclina sinensis, Cadmium, Semi-lethal concentration (LC50), Safe concentration, Accumulation
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