| 摘要: |
| 采用单因子实验设计方法, 进行了饲料中添加铁对美国红鱼生长(增重率、存活率、特定生长率和饲料效率)、免疫反应(血清溶菌酶活性、总补体活性和超氧化物歧化酶活性(Superoxide dismutase, SOD)和组织中铁积累量影响的研究。结果表明, 各饲料组美国红鱼的成活率(93.33%—95.00%)无显著性差异(P>0.05); 饲料铁含量在327.2、368.1mg/kg 时饲料效率显著高于285.3、303.5、443.7mg/kg 组(P<0.05); 随着饲料中铁含
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| 关键词: 美国红鱼, 铁, 生长, 免疫 |
| DOI：10.11693/hyhz200905022022 |
| 分类号: |
| 基金项目:国家科技支撑计划课题, 2007BAD29B04 号; 广东省科技计划重大专项, 2007A03260004 号; 广东省科技计划项目,2007B020708013 号; 广东省重大科技兴海(兴渔)项目, A200501G01 号 |
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| EFFECTS OF DIETARY IRON ON GROWTH AND IMMUNE RESPONSE OF RED DRUM SCIAENOPS OCELLATUS |
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ZHOU Li-Bin1, WANG An-Li2, MA Xi-Lan3, ZHANG Wei2, ZHANG Hai-Fa4
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1.College of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Department of Life Science, Huizhou University and Institute of Biotechnology;2.College of Life Science, South China Normal University, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education;3.Department of Life Science, Huizhou University and Institute of Biotechnology;4.Guangdong Daya Bay Fisheries Development Center
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| Abstract: |
| An experiment was conducted to study the effects of dietary iron on growth (weight rate, feed efficiency ratio, specific growth rate (SGR) and survival rate), immune response [lysozyme activity, total complement activity and superoxide dismutase (SOD) activity] and body distribution of iron concentration of red drum (Sciaenops ocellatus Linnaeus). Five iso-nitrogenous and iso-energetic diets were formulated to contain 285.3, 303.5, 327.2, 368.1 and 443.7 mg Fe per kg diet, supplied as FeSO4·7H2O, and fed in 15 floating netcages (1.5×1.0×1.0m) to 20 fish [initial weight: (173.41±4.85)g] twice a day (09:30 and 16:30) for 8 weeks, the water temperature was set from 26.5 to 32.5℃, salinity from 28 to 29 and dissolved oxygen was approximately 6mg/L during the experimental period. Results indicate that no significant differences in survival rate (93.33%—95.00%) were found among dietary treatments (P>0.05); feed efficiency ratio in 327.2 and 368.1mg/kg treatments were significantly higher than those in 285.3, 303.5 and 443.7mg/kg treatments (P<0.05). Weight rate and specific growth rate in 327.2 and 368.1mg/kg treatment were higher significantly those in other treatments (P<0.05), regression curve analysis shows that the optimum dietary iron requirement for red drum, using SGR as response criteria, was 330mg/kg diet. No significant differences in iron concentration of muscle were found among dietary treatments (P>0.05). Iron concentration in liver increased significantly with increasing dietary iron from 285.3 to 443.7mg/kg (P<0.05). The maximal level of serum lysozyme activity was in 368.1mg/kg treatment, significantly higher than those in other treatments (P<0.05). Serum total complement activity in 327.2, 368.1 and 443.7mg/kg treatments was significantly higher than those in 285.3 and 327.2mg/kg treatments (P<0.05). Serum SOD activity in 327.2 and 368.1mg/kg treatments were higher, but no significant differences in serum SOD activity were found among dietary treatments (P>0.05). Based on the information above, the optimal dietary iron content for optimum growth performance and immune response in red drum is about 368.1mg/kg of dietary iron.
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| Key words: Red drum Sciaenops ocellatus Linnaeus, Iron, Growth, Immune response |
