| 摘要: |
| 2004年1、4、8、10月在大亚湾海域的4个站点采用自行设计和制作的无扰动沉积物采样器采集沉积物样品，通过使用AIT技术(乙炔同时抑制硝化与反硝化作用)，进行实验室同步恒温受控模拟培养实验，并同时测定沉积物上覆水的温度、盐度、DO和pH值，沉积物的Eh值和有机质含量，间隙水的NO3-、NO2-和NH4+浓度，研究沉积物中的硝化与反硝化速率及其影响因素。结果显示大亚湾沉积物的硝化速率范围为[(0.00-4.68)±0.87]μmol/(m2*h)；反硝化速率范围[(0.00-2.88)±0.41]μmol/( m2*h)；硝化与反硝化作用之间存在耦合，比例范围为0%-100%。沉积物的硝化、反硝化速率和耦合比例与上覆水的温度、DO含量，及沉积物中的有机质含量和Eh值密切相关，人类活动对沉积物的硝化与反硝化作用有明显的影响。 |
| 关键词: 大亚湾 沉积物 硝化 反硝化 影响因素 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目,40676073号;浙江省自然科学基金资助项目
,Y505365号;海南省自然科学基金资助项,40651号 |
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| NITRIFICATION AND DENITRIFICATION IN SEDIMENT OF THE DAYA BAY |
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XU Ji-Rong1,2,3, WANG You-Shao1, YIN Jian-Ping1, SUN Cui-Ci1, ZHANG Feng-Qin1, WANG Qin-Ji1, HE Lei1, DONG Jun-De4
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1.South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301;2.Faculty of Architectural Civil Engineering and
Environment of Ningbo University, Ningbo, 315211;3.Tropical Marine Biological Experimental Station of Chinese Academy of Sciences, Sanya,
572000;4.Tropical Marine
Biological Experimental Station of Chinese Academy of Sciences, Sanya,
572000
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| Abstract: |
| Seasonal nitrification and denitrifiication rates in the sediments at 4 stations in Daya Bay were studied from January to October 2004. Samples were cored in 4 sites (named S1, S3, S9 and S11). Nine sediment cores at each site were collected without disturbance with homemade corer. The corer is an acrylic transparent tube, 5cm in diameter and lm long for easy observation. Of the total 9 cores of each site, 6 were used for measuring nitrification and denitrification rates with AIT (acetylene (C2H2) inhibition technique), and other 3 cores were used for measuring temperature (using YSI method), oxidation-reduction potential (Eh), and concentrations of NH4+; and NO2-+ NO3-; in pore water of sediment, respectively. Dissolved oxygen (DO) was determined in Winkler titration, NO4+ and NO2-; NO3- with Technicon Auto-Analyzer AA II; N2O concentration by modified gas chromatographing by Shimadzu 9A gas chromatographer fitted with a 63Ni electron capture detector.
The seasonal average rates of nitrification and denitrification ranged from 0.00 to 4.68±0.87 and (0.00-2.88) ±0.41 μmol/(m2*h), respectively. The nitrification and denitrification counterbalanced in proportion during the seasons between 0%-100% depending on temperature, redox potential, content of organic material in sediment, and DO concentration in overlay water. Human being activities were found to have affected the distribution of DIN (dissolved inorganic nitrogen), organic matter distribution, and the rates of nitrification and denitrification in sediments of the bay. The study shows on the other hand that the process of nitrification/denitrification in the area was weak but complicated. Further study on nitrification/denitrification in this area is suggested. |
| Key words: The Daya Bay, Sediment, Nitrification, Denitrification, Influence factors |
