| 摘要: |
| 采用凝胶过滤层析、SDS．PAGE电泳、Bradford法和苯酚一硫酸法等生物化学方法对菲律宾蛤仔中的蛋白聚糖(PG1和PG2)进行分离纯化，研究其理化性质。采用MTT方法研究蛋白聚糖对人肝癌细胞SMMC-7721和人正常肝细胞HL-7702生长的影响。通过小鼠体内实验研究蛋白聚糖的抗肿瘤活性和对荷瘤小鼠免疫功能的影响。结果表明，PG1分子量大约为20kDa，多糖含量为70.09％，蛋白质含量为15.4％。PG2的多糖含量为82.53％，蛋白质含量为7.26％。PG1在200 g/ml浓度时对SMMC．7721的生长抑制率为73.30％，随浓度的升高，抑制率并未发生明显改变。PG2对SMMC-7721的生长抑制作用呈剂量依赖性，在500μg/ml的浓度下，其抑制效果与PG1的抑制效果相当。在同样浓度下，PG1对人正常肝细胞的生长和功能没有明显影响。PG1呈剂量一效应关系明显增加荷瘤小鼠脾指数，促进荷瘤小鼠脾淋巴细胞转化，且没有降低体重及其他毒副作用。PG1对小鼠S180肉瘤的抑制作用可能是通过直接杀伤肿瘤细胞的细胞毒作用和提高小鼠细胞免疫水平来实现。 |
| 关键词: 蛋白聚糖，菲律宾蛤仔，SMMC-7721，HL-7702，S180 |
| DOI： |
| 分类号: |
| 基金项目:国家“十五”科技攻关项目资助，2001BA708B04-07号。 |
|
| PRELIMINARY STUDY ON THE ANTITUMOR ACTIVITIES OF PROTEOGLYCAN FROM RUDITAPES PHILIPPINARUM |
|
ZHANG Li
|
|
College of Marine Life Sciences and Technology，Ocean University of China
|
| Abstract: |
| Marine animals provide US a wide variety of structurally unique，biologically significant substances for human being’S benefits．Sponges，bryozoans，and ascidians are among the most promising groups．The compounds with antimicrobial and antitumor activity have been frequently found in marine mollusks．Ruditapes philippinarum，belonging to Ruditapes，Veneridae，Bivalvia，is frequently taken as tasty seafood and also atraditional medicine in the Far East for its nutrients and bioactivities for treating internal fever，infections and tumescence．
So far no report is available on the antitumor effect of proteoglycan extract from R．philippinarum both in vitro and in vivo．In this study，we studied the antitumor activities of purified extract from R．philippinarum flesh using tumor—bearing mice and cell lines． Two proteoglycans(PG1，PG2)were purified from hot water extract of R．philippinarum．PG1 was a single Mw 20000 proteoglycan，containing 70．09％ of polysaccharide and 15．4％ of protein．PG2 contained 82． 53％ of polysaccharide and 7．26％ of protein．The inhibition ratio for SMMC-7721 ceils of PG1 at concentration of
200Wg／ml was 73．30％，and increasing concentration did not enhance the inhibitory effect obviously． PG2 inhibited SMMC-772 1 cells proliferation in a dose．dependent manner，and it achieved the same inhibitory effect at concentration of over 500I~g／ml as PG1 achieved at 200I~g／m1．PG1 did not inhibit the growth of human hepatocyte cell HL-7702 when tested in the same concentrations shown to be cytotoxic towards tumor cells．
PG1 exhibited antitumor activity against Sarcoma 180 in KM mice at the dosage of 125-500mg／kg with an inhibition ratio of 24．74％一42．27％，and could enhance the immune function of tumor．bearing mice． The authors believed that the antitumor effect of PG1 is probably host．mediated and cytocida1 |
| Key words: Proteoglycan,Ruditapes philippinarum,SMMC-7721, HL-7702,S180 |
