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| 温度对南极衣藻ICE-L(Chlamydomonas sp. ICE-L)谷胱甘肽含量及其相关酶活性的影响 |
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丁燏1,2,3, 缪锦来4, 王全富4, 阚光锋4, 郑洲4, 李光友4
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1.中国海洋大学海洋生命学院 青岛266003;2.广东海洋大学水产学院;3.湛江524025;4.国家海洋局第一海洋研究所海洋生物活性物质重点实验室 青岛266061
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| 摘要: |
| 采用分光光度法研究了在不同温度下(包括最适温度、低于或高于最适温度)南极衣藻ICE-L(Chlamydomonas sp.ICE-L)谷胱甘肽(GSH)含量、蛋白含量、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽还原酶(GR)和谷胱甘肽硫转移酶(GST)活性,同时分析了已适应高温(16℃)的衣藻重新适应低温(8℃)时这些指标的变化,以期阐明谷胱甘肽系统与南极冰藻低温适应性的关系。结果表明,当温度低于对照组(最适温度8℃)时,蛋白含量降低,而GSH含量、GPx、GST和GR活性上升,已适应高温的衣藻重新适应低温时也出现类似的结果。但当温度高于最适温度时,GSH含量、GST和GPx活性下降,而蛋白含量和GR活力有上升的趋势,GR活力增长幅度比低于最适温度时的变化小。由此可见谷胱甘肽系统在南极衣藻低温适应过程中,GSH、GPx、GR、GST与低温适应呈正相关,同时除GR外其他因子与南极衣藻高温适应呈负相关,GSH及其相关酶在南极冰藻低温适应中具有重要作用。 |
| 关键词: 南极衣藻ICE-L,谷胱甘肽(GSH),谷胱甘肽过氧化物酶(GPx),谷胱甘肽还原酶(GR),谷胱甘肽硫转移酶(GST),低温适应 |
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| 基金项目:国家自然科学基金资助项目,40206022号 |
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| TEMPERATURE EFFECT ON GSH AND GSH-RELATED ENZYMES OF ANTARCTIC ICE MICROALGAE ICE-L (CHLAMYDOMONAS SP. ICE-L) |
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DING Yu,MIAO Jin-Lai,WANG Quan-Fu,KAN Guang-Feng,ZHENG Zhou,LI Guang-You
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1.Marine Life College of Ocean University of China, Qingdao, 266003;2.Fisheries College of Guangdong Ocean University, Zhanjiang, 524025;3.Key Lab of Marine Bioactive Substances, First Institute of Oceanography, State Oceanic Administration, Qingdao, 266061
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| Abstract: |
| Glutathione S-transferase, Acclimation to low temperature Antarctic organisms live in a very harsh natural environment characterized by low temperature, high dissolved oxygen concentration, presence of ice, strong seasonal changes in light intensity, strong radiation, high salinity, dryness and less food source, the conditions that could facilitate the produce o f reactive oxygen species (ROS). GSH defense system is involved in the control of redox balance state, anti-oxidation, and protecting life from injury of ROS. In this paper, a possible GSH system of Chlamydomonas sp. ICE-L of adaptation to low temperature has been investigated by evaluating GSH and GSH-related enzymatic responses at different temperatures using spectrophotometer methods, as well as re-acclimation of Chlamydomonas sp. ICE-L that had acclimatized the temperature that higher than the optimal temperature. The results showed that GSH level, GPx, GST and GR activities were higher at 0°C and -10°C than 8°C , but the protein level decreased. The maximum and minimum of GPx activity were 971.30U and 529.90U respectively at 0°C, and 722.70U and 212.00U respectively at -10°C. But the maximum of GPx was only 242.51U at 8°C. Like the GPx, GST activities were all higher during the whole test time at -10°C and 0°C than at 8°C, and the maximum of it at -10°C and 0°C was about 5 times of that at 8°C. The peak of GR activity was at 0.8336U at 0°C and 0.2395U at -10°C, but that of the control was between 0.0398U and 0.0530U. GSH content of the control was between 1.3072 and 1.4950nmol/μg, but that of the experiment groups could reach 2.5212nmol/μg at 0°C and 2.3980nmol/μg at -10°C respectively. The same results appeared in re-acclimation test. Compared to 16°C, all enzymes activities and GSH level increased more and less at any time during the test and went up very significantly at 24h at 8°C. But the protein concentration did not change regularly like enzymes and GSH in re-acclimation test. GSH level, GST and GPx activities dropped when microalgae ICE-L were taken from 8°C to either 12°C or 17°C , but protein level and GR activity would elevate. The minimum of GSH level, GST, GPx were 0.8176nmol/μg, 51.09U, 101.53U at 12°C respectively, and the maximum of GR activities and protein level were 0.2791U and 0.4303μg/μg respectively at 12°C, and 0.3417U and 0.6447μg/μg respectively at 17°C. It is suggested that the GSH defense system is correlated positively to low temperature and other factors, but GR are negatively to high temperature. Aerobiotic organisms produce a great deal of (ROS) at low temperature, because of greater consumption of oxygen, fast metabolization, and fast electron-transporting1GSH is an essential component in Asc-glutathione cycle, and its role in protecting tissues from toxicants has been recognized clearly in other field. GPx catalyses the reduction of H2O2, organic hydroperoxides, and lipid hydroperoxides using GSH as a reducing agent, and thus help to protect the cells against oxidative damage. GST catalyses the conjugation of glutathione to a variety of electrophilic compounds and plays an important role in the inactivation of xenobiotics and their metabolites, and other chemicals. GR is a key enzyme for maintaining the GSH pool in reduced state. The changes in enzymes activities should be correlated to either the expression of enzymes or the activation of the existed enzymes. These parameters may be used to assess the biological impact of temperature on the Antarctic lives and elucidate the mechanism of Antarctic ice microalgae in acclimating to cold. In a word, GSH and GSH-related enzymes play an important role in the adaptation of Antarctic ice microalgae in low temperature. |
| Key words: Chlamydomonas sp. ICE-L, Glutathione, Glutathione peroxidase, Glutathione reductase, |
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