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| 口虾蛄(Oratosquilla oratoria)同工酶的组织特异性及生化遗传分析 |
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王春琳1,2, 母昌考1, 丁爱侠1, 王 武2
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1.宁波大学生命科学与生物工程学院;2.上海水产大学生命科学与技术学院
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| 摘要: |
| 采用聚丙烯酰胺凝胶垂直平板电泳方法，研究分析了口虾蛄(Oratosquilla oratoria)肌肉、眼球、腹鳃、心脏和肝胰腺5种器官或组织中LDH、ADH、MDH、SDH、MEP、POD、AMY、GDH、SCD、EST、GLDH和SOD共12种同工酶的表达情况。结果表明，口虾蛄同工酶的表达具有明显的组织特异性。对12种同工酶进行生化遗传分析，获得了基本酶谱。12种同工酶共记录出35个基因座位，其中11个基因座位Ldh-3、Adh-1、Sdh-2、Est-1、Est-2、Est-3、Mdh、Mep-7、Gldh-1、Gldh-2和Scd-1为多态，其多态座位比例为31.43％。 |
| 关键词: 口虾蛄, 同工酶, 组织特异性, 生化遗传分析 |
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| 基金项目:国家农业科技成果转化资金项目，02EFN213310651号；浙江省自然科学基金资助项目，302442号 |
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| TISSUE SPECIFICITY AND BIOCHEMICAL GENETIC ANALYSIS OF ISOZYME ON CRUSTACEAN ORATOSQUILLA ORATORIA |
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WANG Chun-Lin1,2, MU Chang-Kao1, DING Ai-Xia1, WANG Wu2
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1.Faculty of Life Sciences and Biotechnology，Ningbo University;2.Faculty of Life Sciences and Technology，Shanghai Fisheries University
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| Abstract: |
| Oratosquilla oratoria is an economically valuable marine crustacean in northeast Asia, such as China, Japan and Korea. Prompted by the absence of any existing isozyme studies on O.oratoria, this paper studied the genetic expression of isozyme of O.oratoria. Thirty-six live O.oratoria were collected from Yuehu market of Ning-bo, China during 2001 and 2002. In total, one hundred and ei ghty biochemi cal samples from five tissues and organs (eye, muscle, ventral gill, heart and hepatopancreas) were taken and, either stored immediately in ultra low temperature freezer. or tested directly in situ. The samples were washed with double distilled water and blotted with disinfected filter paper before storage or testing. Twelve isozymes (LDH, MDH, ADH, MEP, SDH, SCD, SOD, GDH, GLDH, AMY, EST, POD) in a buffer system of TG (Tris-Gly, pH8.3) were tested using vertical polyacrylamide gel electrophoresis (PAGE). Expression in 5 tissues and organ-tissues was also tested. Results showed that isozymes expression contained clear tissue specificity, indicating that variations between different tissues of O.oratoria was a common phenomenon. The basic function of muscle is to move; therefore LDH showed a strong activity in muscle. The function of ventral gills is to respire, MDH-making causing weak LDH’s activity weak in ventral gills is much more strongly expressed in muscle and heart than in other tissues or organs, similar results to those for Penaeus chinensis. Biochemical genetics were analyzed in terms of their locus number, structure, alleles, etc. On the basis of these results, basic electrophoretic patterns of these twelve isozymes were obtained. Altogether, 35 gene loci were recorded, among which ll gene loci included Ldh-3 (alleles, 100, 110), Adh-1(alleles, 100, 105), Sdh-2(alleles, 95, 100, 105), Est-1(alleles, 100, 110), Est- 2(alleles, 100, 110), Est- 3(alleles, 100, 105), Mdh(alleles, 90, 100), Mep- 7(alleles, 75, 100), Gldh-1(alleles, 100, 110), Gldh-2(alleles, 100, 110)and Scd-1(alleles, 100,115) were polymorphic. In addition, the mean proportion of polymorphic loci was 31.43%, higher than that of P.chinensis(15% or 20%). Results of this study suggest that the current variation state of O.oratoria is good. We must protect well this valuable resource from being damaged. |
| Key words: Oratosquilla oratoria, Isozyme, Tissue specificity, Biochemical genetic analysis |
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