| 摘要: |
| 由渤海海泥中分离得到300 株菌株， 从中筛选获得海洋生境芽孢杆菌B- 9987 菌株，其胞外物质经乙醚萃取、硅胶G 薄层层析、官能团显色反应及紫外扫描， 测其组分; 以不同浓度B- 9987 菌株的抑菌液测定对植物病原真菌的抑制效果。结果表明， B- 9987 菌株的胞外抑菌物质可能是酚类化合物; 该物质对植物病原真菌茄交镰孢菌、大丽轮枝菌、黄枝孢菌、立枯丝核菌的抑制作用， 主要是造成真菌孢子或菌丝末端膨大成球状， 继而胞壁崩解， 原生质外泄。对尖刀镰孢菌和莴苣霜霉菌的抑制作用， 则表现为孢子萌发率降低和菌丝长度明显变短。该抑菌物质可望开发为农用抗生素。 |
| 关键词: 海洋细菌, 抑菌物质, 分离纯化, 植物, 病原真菌, 抑菌机理 |
| DOI： |
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| 基金项目:国家"863"项目资助, 2001AA620402 号; 山东省科技兴海重点项目, 001164604 号 |
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| PRELIMINARY STUDY ON PURIFICATION AND ANTIFUNGAL MECHANISM OF METABOLITE FROM MARINE BACTERIUM B-9987 STRAIN ( BACILLUS SP. ) |
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HE Pei-Qing1, TIAN Li1, LI Guang-You1, ZHAO Xiang-Zeng2
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1.Key Lab of Marine Biological Active Substances, State Ocenic Administration;2.Modle Base of Vegetable Science and Technology
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| Abstract: |
| The strain of B-9987 ( Bacillus sp. ) was isolated from sea mud of Bohai Sea in 1999, its antibiotic substances ( GFB87) are strongly against to various plant pathogenic fungi. The purification of GFB87, was studied by the steps of ethyl ether extraction, TLC detection, color reactions, and ultraviolet scan. The spore suspension of Alterinaria solani, Verticillium dahliae, Cladosporiumf ulrumand, Fusarium oxysporum, Bremia lactucae and the hypha suspension of Rhizoctonia solani were treated by the culture filtrate of B-9987 strain diluted to 2—32 times. Then the treatments were cultured at 25℃, and observed with optics microscope after 2, 3, 4, 5, 6, 8, 12, 20 and 48 hours.
The results were as follow. GFB87 were primarily considered as phenolic compounds. Treated by GFB87 antifugi filtrate, the cells of hypha of R. solani, the spore tubes of A. solani , V. dahlia and C. fulrumand, began to dilate to deformed spherical structure, then the cell walls were broken and the protoplasm leaked out ( Fig. 2: 2, 4, 6) , the fungi lost the ability to grow and multiply. More deformed cells were detected with the increase of incubation time.
The culture filtrate diluted to 2 ) 8 times is available ( antifungi rate, 68% ) 92%) , diluted to twice is the best ( antifungi rate, 92%) . While the inhibited hypha cells of F. oxysporum and B. lactucae became obviously shortened, and their germination rate also decreased. |
| Key words: Marine bacterium, Antifungal substance, Purification, Plant, Pathogenic fungi, Antifungal mechanism |
