| 摘要: |
| 于1994年8月—1995年12月，运用生态毒理学和生物化学实验方法，选用扁藻、叉鞭金藻、三角褐指藻及盐藻4种海洋微藻为材料进行海洋微藻对久效磷的抗性与抗氧化能力相关性的实验。结果表明，久效磷在胁迫过程中，微藻细胞内产生了过量的对细胞有伤害作用的活性氧，4种海洋微藻细胞内活性氧清除酶—超氧化物歧化酶的活性高低依次为盐藻>三角褐指藻>叉鞭金藻>扁藻。比较毒性实验证明:4种海洋微藻对久效磷的抗性大小依次为盐藻>三角褐指藻>叉鞭金藻>扁藻，说明4种海洋微藻对久效磷的抗性与其本身抗氧化能力具一定的正相关性，活性氧对细胞的伤害是久效磷毒害的重要原因之一。 |
| 关键词: 海洋微藻 活性氧 超氧化物歧化酶 抗氧化 |
| DOI：10.11693/hyhz200004012012 |
| 分类号: |
| 基金项目:国家攀登计划B资助项目，PDB-6-7-1号 |
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| RELATIONSHIP BETWEEN MONOCROTOPHOS RESISTANCE AND ANTIOXIDIZATION IN FOUR SPECIES OF MARINE MICROALGAE |
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TANG Xue-xi, LI Yong-qi
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College of Marine Life Sciences, Ocean University of Qindao, Qingdao266003
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| Abstract: |
| During Aug. 1994–Dec.1995, an experiment was carried out to investigate the relationship between monocrotophos resistance and antioxidation in four species of marine microalgae using ecotoxicology and biochemistry methods. The results showed that excess active oxygens were induced in algal cells under monocrotophos stress, and the active oxygen caused the damage of alga cells. The order of superoxide dismutes (SOD)’s activities for scavenging active oxygen in four species was: Dunaliella sp.> Phaeodactylum tricornutum > Dicrateria sp. > Platymonas sp.; and monocrotophos resistance of the four species was in the same order Dunaliella sp.> Phaeodactylum tricornutum > Dicrateria sp. > Platymonas sp.. All these indicated that close relationship between monocrotophos resistance and antioxidization in the four species of marine microalgae were obvious, and damage of the active oxygens was one of the main reasons for monocrotophos-induced toxic damage to the four species of marine microalgae. |
| Key words: Marine microalgae, Active oxygen, Superoxide dismutase, Antioxidization |
