| 摘要: |
| 1995年5月－1996年3月，通过一次（分批）培养实验研究了中肋骨条藻和新月菱形藻部分生化组成和性质随培养介质中硝酸盐和磷酸盐水平的变化。结果表明，在氮限制条件下，藻体叶绿素a含量和碱性磷酸酶活力较低，与生长速率呈正相关；碳水化合物含量先随硝酸盐浓度增加而升高，当叶绿素a含量较低时降低。在磷限制条件下，藻体叶绿素a、碳水化合物含量和碱性磷酸酶活力均升高。碳水化合物的积累较氮限制时显著。各生化指标主要在营养盐限制作用系数ψ为0.8–0.9的范围内发生变化，ψ小于0.8为限制状态。中肋骨条藻和新月菱形藻生化指标随营养盐水平的变化有一些差异。氮限制对中肋骨条藻影响较小；新月菱形藻对有机磷利用能力较强，磷限制影响较小，而易受氮限制。 |
| 关键词: 营养盐 浮游植物 叶绿素a 碳水化合物 碱性磷酸酶活力 |
| DOI：10.11693/hyhz200003002002 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，49476286号 |
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| EFFECTS OF NUTRIENTS ON SOME BIOCHEMICAL CONSTITUENTS AND PROPERTIES OF SKELETONEMA COSTATUM AND NITZSCHIA CLOSTERIUM |
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LI Tie, SHI Zhi-li, LI Jun, ZHANG Jin-liang
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College of Chemistry and Chemical Engineering, Ocean University of Qingdao, Qingdao, Qingdao, 266003
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| Abstract: |
| Variations of some biochemical constituents and properties of the two marine diatoms Skeletonema costatum and Nitzschia closterium with nutrients concentrations of culture medium, and their relationship with growth rate are studied in batch culture from May 1995 to March 1996. The biochemical factors include Chlorophyll-a, carbohydrate and the activity of alkaline phosphatas.
The results indicate that the contents of Chlorophyll-a in the algal cells are mainly influenced by nitrate, and the contents of carbohydrate are obviously affected by Chlorophyll-a. Under the condition of nitrogen limitation, the contents of Chlorophyll-a and the activity of alkaline phosphatas in the algal cells are low and are positively correlated with relative growth rates (μ). The content of carbohydrate in the algal cells increases accumulation, then decreases because of the low contents of Chlorophyll-a. Under the conditions of phosphorus limitation, the contents of Chlorophyll-a, carbohydrate and the activity of alkaline phosphatas increase and are negatively correlated with μ. Carbohydrate accumulate more than that under the condition of nitrogen limitation.
The biochemical factors vary distinctly in the range of φ from 0.8 to 0.9. It is surplus when φ > 0.9, limited while φ < 0.8.
The biochemical factors of the two species vary differently with nutrients. S. costatum’s uptake ability of nitrogen is high so nitrogen limitation has little effect on it. The uptake ability of phosphorus and the ability of utilizing organic phosphorus by N. closterium are high, and it is affected little by phosphorus limitation, but is likely to be influenced by nitrogen insufficiency. |
| Key words: Nutrients, Phytoplankton, Chlorophyll-a, Carbohydrate, The activity of alkaline phosphatas |
