| 摘要: |
| 采用随机扩增多态性DNA(RAPD)技术进行了连续3年(1995–1997)共70尾来源于长江水系中华鲟样本遗传分析。共用了40个10bp的随机引物,在26种可供分析的引物中,只有OPK01、OPK02、OPK03、OPK09、OPK14和OPK08RAPD–PCR产物有多态现象,多态引物占23%。26个引物中共扩增出108条稳定的DNA带。其中12条带为多态带,多态座位比例为11.1%。个体间遗传距离变动为0.9510–1.0000,平均为0.9743。1995、1996和1997年的遗传多样性指数(H)分别为0.0327、0.0312和0.0354。3年样本的综合遗传多样性指数(H)为0.034。比较而言,中华鲟天然群体核DNA水平的遗传多样性仍较低。 |
| 关键词: 中华鲟,RAPD,遗传结构,遗传多样性,遗传保护 |
| DOI：10.11693/hyhz200001001001 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 39600112号 |
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| RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) AND GENETIC DIVERSITY OF CHINESE STULRGEON (ACIPENSER SINENSIS) |
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ZHANG Si-ming1, DENG Huai1, YAN Yong1, WANG Deng-Qiang1, WU Qing-Jiang2
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1.Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou, 434000;2.Institute of Hydriobiology, The Chinese Academy of Sciences, Wuhan, 430072
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| Abstract: |
| Chinese sturgeon(Acipensert sinensis), regarding as an “living fossil”, has a high value not only in science but also in commerce. However, duo to habitat destruction, overfishing and pollution etc. the resource abundance has been depleting. In order to monitor the change in resources and develop a sound management program studies on population structure and gene diversity is necessary. Our previous investigation using protein electrophoresis showed that genetic diversity of Chinese sturgeon at protein level was poor. In order to further address the genetic variability Random Amplified Polymorphic DNA (RAPD) was used to screen nuclear genome variation of natural population in Chinese sturgeon from the Yangtze River.
Totally 70 samples were analyzed by RAPD, and among the 70 samples 40, 17 and 13 samples were collected from year 1995,1996 and 1997, respectively. 40 decamer primers were applied for analysis and only 26 primer gave stable result. Among 26 primers studied only 6 primers (23%) such as OPK01, OPK02, OPK03, OPK09, OPK14 and OPQ08 produced polymorphic bands. Total 108 bands (locus) were obtained by using 26 primers. A primer produced 4.2 bands on average. Out of the 108 bands (locus), 12 bands are polymorphic and percentage of loci (P) was about 11.1%. Genetic distance between individuals is 0.9743 on average, ranging from 0.9510 to 1.0000. The genetic diversity H=–Σπilnπi/N which was derived from Shannon’s index of phenotypic diversity Ho=–Σπilnπi were introduced. Total genetic diversity from three year-pooled samples was 0.0334. The genetic diversity for year 1995, 1996 and 1997 were 0.0327, 0.0312 and 0.0354, respectively. Comparatively, the nuclear genome variability is poor, consistent with the data obtained from prom protein electrophoresis. The low genetic variability at nuclear level might take place a long time ago. The construction of the Gezhouba dam on the Yangtze River is not a reason for low genetic variation at nuclear genome because the individuals studied were born before the damming. |
| Key words: Chinese sturgeon(Acipensert sinensis), RAPD, Genetic structure, Genetic diversitry, Genetic conservation |
