| 摘要: |
| 盐藻β–胡萝卜素由中国科学院海洋研究所提供（9505028）。用综合法复制鹌鹑脂肪肝动物模型，给予盐藻β–胡萝卜素以观察其预防高脂饲料致鹌鹑脂肪肝的作用。分组喂以不同饲料，对肝脏进行肉眼和病理组织学检查。结果表明，II-VII组的肝系数与I组相比有较明显的升高（P＜0.05）；盐藻β–胡萝卜素在6.25 mg/kg–25 mg/kg剂量范围内能使肝系数降低，肝脏脂肪病变程度减轻，与Ⅱ组比较有统计学意义(P＜0.05，P＜0.01）。实验证明，自盐泽杜氏藻中提取的以顺式结构为主的β–胡萝卜素具有对抗肝脏脂肪病变的作用。 |
| 关键词: 盐藻β–胡萝卜素 脂肪肝 组织病理学检查 鹌鹑 |
| DOI： |
| 分类号: |
| 基金项目:国家“八五”攻关课题资助项目，85-08-07号 |
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| EFFECTS OF BETA-CAROTENE FROM DUNALIELLA SALINA ON FATTY LIVER IN QUAILS |
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WANG Chun-bo1, LAN Xiao-zhen1, ZHANG Lu-ping2, ZHANG Ren-liang1, Zhang Min1, WANG Yu-Zhen1, WANG Hai-qing1, ZHOU Xu-xiang1, ZHANG Jing-pu3
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1.Medical College of Qingdao University, Qingdao, 266021;2.The Second People’s Hospital of Qingdao, Qingdao, 266033;3.Institute of Ocaenology, The Chinese Academp of Scieiences, Qingdao, 266071
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| Abstract: |
| The β-Carotene was extracted from single cell Dunaliella salina in Hainan Island between July and September. The animal model of hepatic steatasis was reproduced using the multiply methods, and antisteatotic effects ofβ-Carotene from Dunaliella salina on quails fed on high cholesterol, high fat and low protein diet were observed. 84 male quails of 8 weeks with a body weight of 100.2±12.8 g were randomly divided into 7 groups: group I was fed with stock diet; group II hypercholesterol diet; group III–VI hypercholesterol diet plus β-Carotene of 6.25 mg/kg, 12.5 mg/kg, 25 mg/kg, 50 mg/kg, respectively; group VII hypercholesterol diet plus Vitamin E 50 mg/kg. All the quails were killed after 8 weeks. The ratio of the liver and body weight were calculated. The livers were examined by light microscopy. The results show that the ratio of liver and body weight were higher in group II–VII than in that of the normal diet group (P＜0.05). The ratio of liver and body weight were lower in III, IV, V group than that in II group (P＜0.05). Hepatic histopathological examinations show that the pathological changes of fatty liver in quails of β-Carotene 6.25 mg/kg–25 mg/kg were alleviated. The percentage of the liver cell with denatured fat was lower than of the hypercholesterol control group (P＜0.05, 0.01). In conclusion, β-Carotene (cis) from Dunaliella salina has antisteatotic effects. |
| Key words: Dunaliella salina, β-Carotene, Hepatic steatosis histopathology, Quails |
