| 摘要: |
| 于1994–1995年,运用急性毒性实验及生化方法对扁藻和杜氏藻的对硫磷毒性效应进行研究。结果表明,对硫磷对扁藻生长的抑制作用明显强于杜氏藻,在对硫磷的胁迫下,两种微藻细胞的超氧阴离子自由基（O2-）相对含量上升,膜透性增大,微粒体膜脂过氧化水平提高。同时,扁藻微粒体膜的磷脂减少,游离脂肪酸含量增加。说明对硫磷可能导致微藻细胞内活性氧增多,从而引起膜损伤,这种膜损伤是膜脂过氧化与脱酯化单独或共同作用的结果。 |
| 关键词: 对硫磷 扁藻 杜氏藻 膜脂 过氧化 脱酯化 |
| DOI： |
| 分类号: |
| 基金项目:国家攀登计划B资助项目，PDB-6-7-1号 |
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| DAMAGE OF PARATHION ON PLATYMONAS SP. AND DUNALIELLA SP. BY MEMBRANE LIPID PEROXIDATION AND DEESTERIFICATION |
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TANG Xue-xi, LI Yong-qi, HUANG Jian
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College of Marine Life Sciences, Ocean University of Qingdao, Qingdao, 266003
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| Abstract: |
| During 1994–1995, the toxic effect of parathion on two marine microalgae was analyzed with toxicity and biochemistry methods. The results show that the 72h EC50 of Platymonas sp. was 4.6mg/L, while 72h EC50 of Dunaliella sp. was 8.3mg/L; therefore, the growth inhibition of parathion on Platymonas sp. was stronger than that on Dunaliella sp. Under parathion stress, the content of active oxygen increased significantly, membrane permeability enlarged, and membrane lipid peroxidation increased remarkably in two marine microalgae. Under parathion stress, the content of free fatty acid increased and phospholipid content decreased only in Platymonas sp. It was concluded that membrane damage in algal cell resulted from excess active oxygen which was produced by algae under parathion stress. In Platymonas sp., the membrane damage appeared by means of membrane lipid peroxidation and deesterification together; while in Dunaliella sp., it was membrane lipid peroxidation alone that resulted in membrane damage, without any relation to deesterification. |
| Key words: Parathion, Platymonas sp., Dunaliella sp., Membrane lipid, Peroxidation, Deesterification |
