| 摘要: |
| 于1996年6月一1997年5月，采用半叶紫菜华北变种的丝状体（A）（本所提供）和野生半叶紫菜华北变种的叶状体果泡子（B）（1995年3月采于青岛湛山海区），分别接种贝壳丝状体，采用室内培养方法，对壳孢子成熟、放散条件、大小及幼苗的生长发育进行研究。结果表明，A的壳孢子较小，成熟难，放散温度为10℃；幼苗的长度在10－22℃的范围内随温度的升高而增加，幼苗的形态与温度无关，在15一20℃放散单孢子。B的亮孢子较大，成熟较容易，放散温度为14℃；壳孢子苗的形态与温度没有明显的相关性，而且始终未放散单孢子。两种来源的壳孢子苗的形态建成也有明显差别。 |
| 关键词: 半叶紫菜华北变种 丝状体 壳孢子 壳孢子苗 |
| DOI： |
| 分类号: |
| 基金项目:国家攀登计划B资助项目，PD B-6-4号 |
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| ARTIFICIAL CULTURE FROM CONCHOCELIS TO CONCHOSPORELINGS OF PORPHYRA KATADAI VAR. HEMIPHYLLA |
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TANG Xiao-rong, FEI Xiu-geng
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Institute of Oceanology, ne Chinese Acadeny of Sciences, Qingdao, 266071
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| Abstract: |
| Conchocelis of Porphyra katadai var. hemiphylla from indoor culture and wild carpospores from leafy thalli collected on the Zhanshan region, Qingdao seashore in March 1995 and kept on -20°C for about fifteen months, were used and their Conchospore maturing and releasing conditions, size, growth and development of Conchosporeings were studied in order to identify the life history of P. katadai var. hemiphylla. The results show that conchocelis from indoor culture was more difficult to form and release conchospores than that from wild carpospores. Sporangial branchlets were formed in large quantity at 30-31°C, releasing conchospores at 10°C. Average diameter of conchospores was (11.3±1.3)μm. The lengths of Conchosporelings increased with temperature at 10-22°C. A temperature of 15-22°C were optimal for sporeling normal growth. The shapes of sporelings were not related to the temperature. Conchosporelings shed monospores at 15-20°C. Conchocelis from wild carpospores formed sporangial branchlets in large quantity at 26-28°C and released conchospores at 14°C. Average diameter of conchospores was (13.1±1.3)μm. Conchosporelings grew quickly at 10°C and their shapes were not related to the temperature significantly. Conchosporelings from two sources grew normally at lower temperature and no monospore was shed. For the morphogenesis of conchosporelings, conchospores from indoor culture germinated into sporelings with no more than 10 cells being lined along the germination direction. Certain cells on the position of apex or middle were divided longitudinally to form two lines. Conchospores from wild carpospores germinated into two cells in the direction of germination. Afterwards, there were two modes for the sequential cell division. One is the apical cell which was divided longitudinally to form two new cells, which occupied about 50% of the total division modes. The two newly produced cells were further divided to form the two halves of an adult thallus. The other mode was that after the first division, the division direction did not change until a certain cell divided longitudinally to form a multi-lined thallus when the cell number of a conchosporeling was about 10. In conclusion, there exist strains in Porphyra katadai var. hemiphylla, Porphyra katadai var. hemiphylla has the ability to produce monospores in certain conditions. |
| Key words: Porphyra katadai var. hemiphylla, Conchocelis, Conchospore, Conchosporelings |
