| The shrimp (Penaeus chinensis), being 11-13cm in body length and 15-20g in body weight, was collected from Jiaozhou Bay in September-October 1994 and 1995, respectively, and then cultured in laboratory tank. The shrimps were kept in the tank (100× 75× 80 cm3) for 7-10 days before the experiment and 2/3 of aerated seawater were changed daily, and were fed with diet supplemented with Pollen, Vc,VB6 and No.1 or No.2 (Tab.1) Chinese herbal medicine respectively, another group were injected with No.1 or No.2 Chinese herbal medicine. The haemolymph was taken from the fed shrimps, which were cultured for 17 or 25 days, and the injected shrimps which were cultured for 3 days to test change of their nonspecific immunity, i. e. bacteriolytic activity and agglutinative activity. The results were as follows:
1. When the shrimps were fed with diet containing Pollen, Vc,VB6, their nonspecific immunity, i.e. bacteriolytic activity and agglutinative activity could not be improved (Tab. 2).
2. The bacteriolytic activity in the serum was, respectively, 0.167 and 0.73 for the shrimps fed with diet containing No.1 Chinese herbal medicine, measured for 17 and 25 days after feeding, it was 0.135 and 0 for the shrimps fed with diet containing No.2 Chinese herbal medicine measured on the 17 and 25 days, respectively (Tab.3).
3. The agglutinative activity in the serum was, respectively, 64 and 128 for the shrimps fed with diet containing No.1 Chinese herbal medicine, measured for 17 and 25 days after feeding, it was 32 and 8 for the shrimps fed with diet containing No.2 Chinese herbal medicine measured on the 17 and 25 days, respectively (Tab. 3).
4. The bacteriolytic activity in the serum was 0.65 and agglutinative activity titer in the serum was 64, for the shrimps, which were injected 100 μl No.1 Chinese herbal medicine 3 days latter. The bacteriolytic activity in the serum was 0, and agglutinative activity titer in the serum was 4, for the shrimps, which were injected 100 μl No.2 Chinese herbal medicine 3 days later (Tab.4).
5. The color of haemolymph was blue for the shrimps fed with diet containing No.1 Chinese herbal medicine for 17 and 25 days, it was same as the control (Tab.3). The shrimps were injected with 100 μl No.l Chinese herbal medicine 3 days later, the haemolymph colour was same as the control (Tab. 4). The colour of haemolymph was blue for shrimps fed with diet containing No.2 Chinese herbal medicine for l7 days, but the haemolymph colour was orange for the shrimps fed with diet containing No.2 Chinese herbal medicine for 25 days (Tab. 3). The shrimps were injected with 100 μl No. 2 Chinese herbal medicine 3 days later, and the haemolymph colour was also orange (Tab.4).
The above results showed that No.1 Chinese herbal medicine could improve nonspecific immunity of the shrimps, and increase their disease resistance. |
