| 摘要: |
| 于1990年3月用β－琼胶酶对多管藻的冷水提取多糖进行酶解，酶解液通过DEAE－SephadexA25和Bio－Gel P6色谱柱分离，分离出2个带电荷的寡糖A-Ⅰ和A-Ⅱ．经1H-NMR和13C-NMR光谱法分析，确定结构式分别为61，22-二-O-甲基-63-硫酸基-新琼四糖和61，22-二-O-甲基-63，65-二硫酸基-新琼六糖，证明它们构成多管藻多糖分子的单位组分。 |
| 关键词: β－琼胶酶 多管藻 带电荷琼胶寡糖 新琼四糖 新琼六糖 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，3870137号 |
|
| STUDIES ON THE STRUCTURE OF OLIGOMERS FROM THE POLYSACCHARIDE OF POLYSIPHONIA URCEOLATA (RHODOPHYTA) USING β-AGARASE AND NMR SPECTROSCOPY |
|
Gao Hongfeng, Ji Minghou, Cao Wenda, Han Lijun
|
|
Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
|
| Abstract: |
| The polysaccharide extracted by cold water from Polysiphonia urceolata collected on March 1990, in Qingdao, was hydrolyzed using β-agarase from Pseudomonas atlantica. The mixture after hydrolysis was precipitated by adding 95% ethanol solution, then the upper clear solution was evaporated in vacuo. This ethanol concentrate of enzyrnic hydrolyzate was chromatographed on DEAE-Sephadex A25 and then Bio-Gel P6 columns. Two charged agarooligomers A-I and A-II were isolated from the eluents. Their degrees of polymerization were estimated by 1H-NMR spectra to be 2 and 3, respectively. Two oligomers gave clear resonance signals at 3-41 ppm and 3.52 ppm in 1H-NMR, which showed the presence of methyl groups on C2 of L-galactose and C6 of D-galactose, respectively. A strong signal at 4.18 ppm indicated that a sulfate group links with C6 of D-galactose. The structure of the oligomers A-I and A-II was deduced by the assignment of chemical shifts in 1H-and 13C-NMR spectra to be 61, 22-di-nethyl-meoagarotetraose-63-sulfate and 61, 22-di-methyl-neoagarohexaose-61, 65-disulfate, respectively, which constitute the unit component of the polysaccharide of Polysiphonia urceolata. |
| Key words: β-agarase, Polysiphonia urceolala, Charged agarooligomer, Neoagarotetraose, Neoagarohexaose |
