| 摘要: |
| 于1991年9月在青岛市沙子口镇对虾养殖场采集中国对虾病虾，采用酒石酸钾－甘油密度梯度离心法从病虾肝胰腺中分离出一种呼肠孤科病毒，并通过电子显微镜观察和提取分析病毒基因组核酸等方法进行初步鉴定。结果显示，该病毒在酒五酸钾-甘油密度梯度离心纯化时与肝胰腺细小病毒混合存在；病毒颗粒为有包膜的正二十面体立体对称结构；带包膜的完整颗粒直径在64－66nm；裸露核衣壳平均直径为53.2nm；病毒基因组由分成10个节段的双链RNA构成。目前还没发现与该病毒有明确关系的组织和细胞病理变化。 |
| 关键词: 中国对虾 呼肠孤病毒 混合感染 鉴定 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39000083号 |
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| PRIMARY IDENTIFICATION OF A REOVIRUS INFECTED PENAEUS CHINENSIS TOGETHER WITH HEPATOPANCREATIC PARVOVIRUS |
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Xue Qinggang1, Gong Yunhao2, Wang Wenxing1
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1.First Institute of Oceanography, SOA, Qingdao 266003;2.National Animal Quarantine Institute, Ministry of Agriculture, Qingdao 2660032
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| Abstract: |
| A reovirus was separated in 192 - 1993 from the hepatopancreas of the diseased Penaeus chinensis collected in September, 1991 at the Shazikou Shrimp Farm, Qingdao. After purification by ultracentrifugation through a potassium tartrate-glycerol density gradient, the virus preparation showed 2 different virus particles under the electron microscope. The smaller virus particle, was icosahedral and 23 – 29 nm in diameter, and was identified as the hepatopancreatic parvovirus of Penaeus chinensis. The larger one was an enveloped viron with spherical shape and diameter of 64 – 66 nm. The naked nucleocapsid of the larger virus particle was icosahedral and 51.2 – 56.6 nm (53.2nm in average) in diameter. After extraction from the virus preparation, treatment with DNase, RNase and Nuclease S1, electrophoresis on agarose gel, the genomic nucleic acid was revealed to be completely different from the 4.3kbp single stranded DNA of the hepatopancreatic parvovirus of Penaeus chinensis, but was composed of 10 fragments of double stranded RNA. As the morphological characteristics and the genome properties of the newly separated virus were abviously similar to those of the Reoviridae, the virus should be a member of this family. The histopathological and cytopathological changes that definitely related to the virus have not yet been discovered. |
| Key words: Penaeus chinensis, Reovirus, Mixed infection, Identification |
