| 摘要: |
| 于1989年1月 - 1989年8月采用连续培养和半连续培养方法进行了伪矮海链藻细胞分裂、叶绿素a含量和活体荧光特性与光、营养盐关系的研究。结果表明,细胞分裂、活体荧光、叶绿素a均呈现光照期的增长速率明显高于黑暗期的增长速率的日变化规律,荧光增强比则在光照期开始后或黑暗期结束时出现最高值;光强和营养盐不仅影响各指标日变化的幅度,而且还可改变荧光增强比峰值出现时间。因此,在研究细胞分裂、叶绿素a和荧光特性的昼夜节律时,必须考虑光和营养盐这两个重要因素。 |
| 关键词: 伪矮海链藻 昼夜节律 光 营养盐 叶绿素a 荧光特性 |
| DOI： |
| 分类号: |
| 基金项目:美国海军研究基金资助项目,N14-87-K-0311-1号 |
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| EFFECT OF LIGHT AND NUTRIENT ON THE DIEL VARIATION OF THALASSIOSIRA PSEUDONANA I. CELL DIVISION, CHLOROPHYLL a AND ITS IN VIVO FLUORESCENCE CHARACTERISTICS |
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Yang Xiaolong, Zhu Mingyuan
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First Institute of Oceanography, SOA, Qingdao 266003
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| Abstract: |
| During Jan. to Aug. 1989, continuous and semi-continuous culture method were used to explore the relationships between light, nutrient and the diel variation of cell division, chlorophyll a content, in vivo fluorescence characteristics in Thalassiosira pesdonana. The results indicate that cell division, in vivo fluorescence, chlorophyll a exibited a more rapid increase during the light period than during the dark period.
The increase of light intensity not only enhanced the specific growth rate of cell division, in vivo fluorescence, chlorophyll a during the light period but also changed the diel pattern of enhanced fluorescence ratio; its maximum value occurred 3 h after exposure to low light intensity, at high light intensity the end of the dark period, maximum values occurred. Nutrients also enhanced the changing amplitude of the diel variations of in vivo fluorescence, enhanced fluorescence ratio, chlorophyll a and changed the timing of daily maxima in enhanced fluorescence ratio.
It can be concluded from the results of experiments that light and nutrient have profound effects on the amplitude and timing of the diel variations in the parameters studied. Thus light and nutrient must be considered in the study of the dield variations of growth and metabolism in phytoplankton. The mechanism of the diel variation of these parameters is also discussed in this paper. |
| Key words: Thalassiosira pesdonana, Diel variation, Light, Nutrient, Chlorophyll a, Fluorescence characteristics |
