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不同浓度氨氮胁迫下长蛸的半致死浓度和急性毒性评估*
陈智威, 许然, 南泽, 郑小东
中国海洋大学海洋生物多样性与进化研究所
摘要:
作为重要的水质参数,高浓度的氨氮对水生动物产生有害影响。氨氮的安全浓度对海水养殖物种至关重要。本文采用静水法, 在盐度30.0±1.0,pH7.8±0.2,温度20.8±0.5℃条件下,以荣成月湖海区的长蛸(Octopus minor)成体为研究对象,分析了水体氨氮对其急性毒性作用。通过预实验获得长蛸成体可耐受的浓度范围,从中选取浓度0mg/l(对照)、87mg/l、124.5mg/l、143.5mg/l、180.3mg/l、204.0mg/l、242.5mg/l 等7个浓度梯度测定长蛸成体耐受性,采用概率单位法、寇氏法和线性回归法分别计算48h、72h和96h的半致死浓度。实验结果显示, 氨氮毒性效应随着浓度和胁迫时间的增长而增加,氨氮胁迫48h、72h和96h的半致死浓度分别为187.93mg/l、154.61mg/l和124.92mg/l( 卡方检验P<0.05);显示氨氮对长蛸成体具有显著毒性作用。与其他水生动物相比,长蛸对于氨氮的耐受性较强,对氨氮毒性的自我调节能力较强,具体的调节机制有待进一步研究。
关键词:  长蛸  氨氮  急性毒性  半致死浓度
DOI:
分类号:
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
附件
ASSESSMENT OF THE MEDIAN LETHAL CONCENTRATION AND ACUTE TOXICITY OF OCTOPUS MINOR UNDER DIFFERENT CONCENTRATIONS OF AMMONIA AND NITROGEN STRESS
Chen Zhiwei, Xu Ran, Nan Ze, Zheng Xiaodong
Institute of Evolution and Marine Biodiversity, Ocean University of China
Abstract:
As an important water quality parameter, high concentrations of ammonia nitrogen have a detrimental effect on aquatic animals. The safe concentration of ammonia nitrogen is critical for marine aquaculture species. In this study, the static biological toxicity test was used to analyzed the acute toxicity of ammonia nitrogen on the Octopus minor adult in the Swan Lake area by calculate the mortality in water salinity 30.0±1.0, pH 7.8±0.2 and temperature 20.8±0.5℃. In this study, an ammonia nitrogen range-finding toxicity test was performed by the death situation of O. minor in pre-experiment. And we used the results obtained in the range-finding experiment, to define sub-lethal ammonia nitrogen exposure treatments and exposed O.minor to ammonia nitrogen concentration of 0mg/l(control)、87mg/l、124.5mg/l、143.5mg/l、180.3mg/l、204.0mg/l、242.5mg/l for 48h, 72h and 96h. Finally, we used probit analysis, Kouchi method and linear regression method to calculate the sub-lethal of 48h, 72h and 96h..The results showed that the toxic effect of ammonia nitrogen increased with the increase of concentration and stress time. The median lethal concentrations of ammonia nitrogen stress at 48h, 72h and 96h were 187.93mg/l, 154.61mg/l and 124.92mg/l, respectively (test P<0.05). It can be considered that ammonia nitrogen has a significant toxic effect on the species. Compared with other aquatic animals, O. minor is more tolerant to ammonia nitrogen. For example, the median lethal concentration of Apostichopus japonicus for 96h is 90.57mg/l, which is lower than that of O.minor. It can be presumed the self-regulating of ammonia nitrogen toxicity is stronger than some aquatic animals, and the specific regulatory mechanism needs further researches.
Key words:  Octopus minor  ammonia nitrogen  acute toxicity  the median lethal concentration
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