| 摘要: |
| 二苯甲酮-3 (BP-3)、4-甲基苄亚基樟脑(4-MBC)和4-甲氧基肉桂酸-2-乙基己酯(EHMC)是三种常用的有机紫外吸收剂, 在水环境中被频繁检出, 对水生生态系统安全构成潜在威胁。为探究三种有机紫外吸收剂对菲律宾蛤仔(Ruditapes philippinarum)鳃组织抗氧化响应和相关细胞凋亡基因的影响, 将蛤仔分别暴露于环境相关浓度的三种紫外吸收剂溶液中, 检测鳃组织抗氧化酶活性和细胞凋亡相关基因转录水平, 并通过第二代整合生物标志物响应法(IBRv2)对三种紫外吸收剂的生物毒性进行比较分析。结果显示, 三种紫外吸收剂短期暴露会诱导抗氧化响应提高抗氧化能力, 而长期高浓度暴露会导致抗氧化能力的降低。BP-3、4-MBC和EHMC可能通过启动线粒体途径和fas介导的死亡受体途径诱导菲律宾蛤仔鳃组织产生细胞凋亡。通过IBRv2分析发现, 在环境常见浓度1 μg/L的暴露水平下, 短期(1 d, 7 d)暴露时, BP-3对菲律宾蛤仔鳃组织表现出的综合毒性效应最强, 而随着暴露时间的延长(28 d), 三种紫外吸收剂表现出的综合毒性效应相近。研究结果为水环境中有机紫外吸收剂的生态风险评估提供了参考数据。 |
| 关键词: 有机紫外吸收剂 菲律宾蛤仔 鳃 氧化胁迫 细胞凋亡 整合生物标志物响应法 |
| DOI：10.11693/hyhz20230600126 |
| 分类号: |
| 基金项目:自然资源部东南生态脆弱区监测修复工程技术创新中心自主研究课题,KY-090000-04-2022-017号;福建省大学生创新创业计划项目,S202210389038号。 |
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| EFFECT OF THREE ORGANIC ULTRAVIOLET FILTERS ON ANTIOXIDANT CAPACITY AND EXPRESSION OF APOPTOSIS-RELATED GENES IN GILLS OF RUDITAPES PHILIPPINARUM |
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ZHANG Wei-Wei1,2, DONG Fei-Long1,2, JING Chen1,2, LIU Shang-Shu1,2, HU Feng-Xiao1,2
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1.College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;2.Key Laboratory of Marine Biotechnology of Fujian Province, Fuzhou 350002, China
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| Abstract: |
| Benzophenone-3 (BP-3), 4-methyl-benzylidene camphor (4-MBC), and 2-ethyl-hexyl-4-trimethoxycinnamate (EHMC) are commonly used organic ultraviolet (UV) filters. Recently, these UV filters have been frequently detected in aquatic environment, posing a potential threat to the safety of aquatic ecosystem. To investigate the effects of the three UV filters on the antioxidant response of Ruditapes philippinarum gill and apoptosis-related genes, R. philippinarum was exposed to the three filters at environmentally relevant concentrations, and the activity of antioxidant enzymes and transcriptional levels of apoptosis-related genes in gills were investigated. Afterwards, the adverse effects of the filters were compared and analyzed using the Integrated Biomarker Response (IBR) (Version 2) method. Results show that the organic UV filters could induce initial antioxidative response to improve antioxidant capacity, while a long-term exposure to high concentrations of the UV filters could decrease the antioxidant capacity. In addition, the three filters could induce apoptosis in the gill tissue of R. philippinarum via mitochondria pathway and death receptor pathway. After exposure to the three organic UV filters at common environmental concentration (1 μg/L) for 1 d and 7 d, BP-3 exhibited the strongest toxic effects on R. philippinarum gill; and the biomarker responses were similar among the three filters in 28 d. Therefore, the toxicity of organic UV filters depends on chemical species, exposure dose, and exposure duration. This study provides reference data for ecological risk assessment of organic UV filters in aquatic environments. |
| Key words: organic UV filters Ruditapes philippinarum gill oxidative stress apoptosis integrated biomarker response |
