| 摘要: |
| 研究分析了嗜水气单胞菌(Aeromonas hydrophila)感染斑马鱼后肠道生理健康和肠道微生物的变化。以斑马鱼为研究对象, 刮伤皮下真皮, 使用105 CFU/mL浓度的嗜水气单胞菌暴露6 h后转入清水, 分别在暴露前、暴露后6 h、12 h、24 h取样。使用鱼特异性酶联免疫吸附试验(ELISA)试剂盒, 检测肠道中紧密连接蛋白ZO-2 (TJP2)含量和乙氧基异戊二烯-O-脱乙基酶(EROD)酶活性, 结果表明, 嗜水气单胞菌暴露引起了斑马鱼肠道生理损伤, 表现为肠道TJP2含量、EROD酶活性在暴露后显著下调。采用16S rRNA高通量测序检测肠道微生物及其菌群结构变化, 嗜水气单胞菌暴露斑马鱼后致病菌不动杆菌属(Acinetobacter)明显增加, OTUs数量明显下降, Alpha多样性降低, 结果说明嗜水气单胞菌引起了斑马鱼肠道菌群失调。肠道中微生物的多样性与改善肠道上皮和黏膜屏障功能紧密相关, 该研究对从肠道健康的角度评价嗜水气单胞菌毒性, 探索嗜水气单胞菌的致病机制具有重要意义。 |
| 关键词: 嗜水气单胞菌 斑马鱼 肠道微生物 Alpha多样性 TJP2 |
| DOI：10.11693/hyhz20221000254 |
| 分类号:S917.1 |
| 基金项目:安徽省农业科学院科研团队计划项目, 2022YL010号; 安徽省现代农业产业技术体系建设专项, 皖农科函[2021] 711号。 |
附件 |
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| AEROMONAS HYDROPHILA CAUSED INTESTINAL PHYSIOLOGICAL DAMAGE AND INTESTINAL MICROBIAL DISORDER IN ZEBRAFISH |
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SUN Wen1,2,3, WANG Yong-Jie1,2,3, BAO Jun-Jie1,2,3, ZHANG Jing1,2,3, CHEN Hong-Lian1,2,3, XIONG Ying-Qi1,2,3
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1.Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;2.Key Laboratory of Aquaculture &3.Stock Enhancement in Anhui Provincial, Hefei 230031, China
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| Abstract: |
| Aeromonas hydrophila is widely distributed in nature, and is a typical opportunistic pathogen of common diseases of human and fish. To understand the mechanism of its infection to fish, zebrafish was taken for this study. The subcutaneous dermis of the sample fish was scratched, and the wounded fish was exposed to A. hydrophila solution at a concentration of 105 CFU/mL for 6 h. Samples were taken at 0, 6, 12, and 24 h after exposure. The changes of intestinal physiological health and intestinal microbiota of the fish were analyzed. Fish specific enzyme-linked immunosorbent assay (ELISA) kit was used to detect the tight junction protein ZO-2 (TJP2) content and ethoxyisoprenoe-O-deethylase (EROD) activity in the intestine. Results show that after the exposure, the intestine of the zebrafish was damaged, and the intestinal TJP2 content and EROD enzyme activity were significantly down-regulated. As revealed by 16S rRNA high-throughput sequencing, the structures of intestinal microorganisms and their microbiota were altered, Acinetobacter was significantly increased, the number of OTUs were significantly decreased, the Alpha diversity was decreased, and the dysregulation of the intestinal microbiota was caused. The diversity of intestinal microorganisms was closely related to the improvement of intestinal epithelial and mucosal barrier function. This study provided a reference for studying the toxicity of A. hydrophila from the perspective of intestinal health and exploring the pathogenic mechanism of A. hydrophila in zebrafish. |
| Key words: Aeromonas hydrophila zebrafish intestinal microbial Alpha diversity TJP2 |
