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引用本文:钟爱华,王迎宾,毕澜方,徐浩磊.溶藻弧菌体外刺激泥蚶(Tegillarca granosa)血细胞后早期转录组研究.海洋与湖沼,2023,54(1):286-295.
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溶藻弧菌体外刺激泥蚶(Tegillarca granosa)血细胞后早期转录组研究
钟爱华, 王迎宾, 毕澜方, 徐浩磊
浙江海洋大学水产学院 浙江舟山 316022
摘要:
泥蚶(Tegillarca granosa)是我国传统养殖贝类,细菌性疾病导致的死亡已成为制约泥蚶养殖业发展的重要因素,血细胞是泥蚶免疫防御的执行者,血细胞能直接吞噬异物,能通过包囊作用将异物包裹,血细胞还在炎症反应和伤口修复中发挥作用。采用RNA-seq测序技术研究了溶藻弧菌体外刺激后泥蚶血细胞3h和6h时转录组动态变化,与未受刺激血细胞相比,3h时1790个基因表达发生显著改变,包括1319个上调基因和471个下调基因;6h时3183个基因表达发生显著改变,包括2629个上调基因和554个下调基因。KEGG通路富集分析发现溶藻弧菌刺激后免疫相关多个信号通路或生物学过程发生显著改变,如吞噬体、细胞凋亡、蛋白酶体、内质网加工、局部黏附等。部分免疫相关基因表达丰度3h时变化不显著而6h时发生显著改变,部分免疫相关基因3h和6h时表达丰度均显著改变。研究结果为泥蚶血细胞早期免疫反应提供了新的见解,为泥蚶抗病机理研究提供了理论基础。
关键词:  泥蚶(Tegillarca granosa)  溶藻弧菌(Vibrio alginolyticus)  血细胞  转录组
DOI:10.11693/hyhz20220500123
分类号:S917.4
基金项目:浙江省教育厅项目,Y202044632号。
附件
THE TRANSCRIPTOMIC ANALYSIS OF IN VITRO HEMOCYTES OF BLOOD CLAM (TEGILLARCA GRANOSA) CHALLENGED WITH VIBRIO ALGINOLYTICUS
ZHONG Ai-Hua, WANG Ying-Bin, BI Lan-Fang, XU Hao-Lei
College of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
Abstract:
Blood clam (Tegillarca granosa) is a traditional cultured shellfish in China. The mortality caused by bacterial diseases has become an important issue restricting the development of clam breeding industry. Hemocytes are the executor of immune defense, and they can directly swallow foreign bodies and wrap them up by encapsulation. Hemocytes also play a role in inflammatory reaction and wound repair. In this paper, RNA sequencing was used to study the dynamic changes of transcriptome of in vitro hemocytes stimulated by Vibrio alginolyticus in 3h and 6 h. Compared with unstimulated hemocytes, the expression of 1 790 genes were significantly changed at 3 h, including 1 319 up-regulated genes and 471 down-regulated genes. In 6 h, the expression of 3 183 genes changed significantly, including 2 629 up-regulated genes and 554 down regulated genes. KEGG pathway enrichment analysis found that many immune-related signal pathways or biological processes changed significantly after V. alginolyticus stimulation, such as phagosome, apoptosis, proteasome, endoplasmic reticulum processing, local adhesion and other pathways. The expression abundance of some immune-related genes did not change significantly in 3 h, but changed significantly in 6 h, and the expression abundance of some immune-related genes changed significantly in 3h and 6 h. This study provided a new insight into the early immune response of the hemocytes of blood clam and a theoretical basis for the study of disease resistance mechanism of blood clam.
Key words:  Tegillarca granosa  Vibrio alginolyticus  hemocytes  transcriptome
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