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引用本文:陈传悦,谢兵,孙闻婧,廖智,严小军,张晓林.白藜芦醇对饥饿条件下厚壳贻贝(Mytilus coruscus)抗氧化能力的影响.海洋与湖沼,2023,54(1):266-275.
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白藜芦醇对饥饿条件下厚壳贻贝(Mytilus coruscus)抗氧化能力的影响
陈传悦1,2, 谢兵1, 孙闻婧1, 廖智1, 严小军1,2, 张晓林1
1.浙江海洋大学海洋科学与技术学院 浙江舟山 316022;2.宁波大学海洋学院 浙江宁波 315211
摘要:
饥饿胁迫引起的肥满度下降及收割延迟是导致贻贝养殖产量下降的主要原因之一。探究白藜芦醇(Resveratrol,RES)对饥饿条件下贻贝抗氧化能力的影响,揭示贻贝对饥饿胁迫的响应机制,对指导贻贝健康养殖具有重要意义。在饥饿胁迫条件下,分别采用10、20、50、100μmol/LRES处理厚壳贻贝,9d后采集贻贝组织样品并进行氧化应激指标检测。结果表明,饥饿胁迫显著增加厚壳贻贝组织中丙二醛(MDA)含量,同时显著降低过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)、碱性磷酸酶(AKP)和酸性磷酸酶(ACP)活性和还原性谷胱甘肽(GSH)含量。RES处理后,厚壳贻贝组织中MDA含量显著降低,但随着RES浓度的增加显著升高;性腺和鳃中CAT、SOD、GSH-PX、AKP、ACP活性和GSH水平、后闭壳肌中CAT、AKP活性和GSH水平以及外套膜中CAT、SOD、GSH-PX、ACP活性和GSH水平均随着RES处理浓度的增加先升高后降低。此外,10或20μmol/LRES处理能显著减轻饥饿胁迫引起的贻贝性腺中滤泡降解、配子退化及鳃丝纤毛脱落及结构受损;且各组贻贝后闭壳肌和外套膜组织形态学无明显变化。研究结果表明饥饿胁迫能显著抑制厚壳贻贝的抗氧化能力,但10或20μmol/LRES处理可减轻饥饿胁迫诱导的脂质过氧化和组织氧化应激损伤,且厚壳贻贝对饥饿胁迫诱导的氧化应激损伤表现出一定的组织差异性。
关键词:  厚壳贻贝  饥饿胁迫  氧化应激  酶活性  组织损伤
DOI:10.11693/hyhz20220500140
分类号:P735
基金项目:舟山市科技局项目,2019F71053号;国家自然科学基金委重点国际(地区)合作与交流项目,42020104009号。
附件
EFFECTS OF RESVERATROL ON ANTIOXIDANT CAPACITY OF MYTILUS CORUSCUS UNDER STARVATION STRESS
CHEN Chuan-Yue1,2, XIE Bing1, SUN Wen-Jing1, LIAO Zhi1, YAN Xiao-Jun1,2, ZHANG Xiao-Lin1
1.Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, China;2.School of Marine Sciences, Ningbo University, Ningbo 315211, China
Abstract:
The starvation stress of Mytilus decreases the fatness and delays the harvest time, which is a main reason for the decline of Mytilus aquaculture yield. To explore the effects of resveratrol (RES) on the antioxidant capacity of Mytilus coruscus under starvation stress, and reveal the response mechanism of the mussels to starvation stress and the role of RES in starvation-stressed mussels for healthy mussel farming, M. coruscus were experimental studied in treatment groups at 10, 20, 50, and 100 μmol/L RES levels under starvation stress. The treated samples were collected after 9 days of treatment and oxidative stress indicators were detected. Results show that starvation stress significantly increased malondialdehyde (MDA) content, and significantly decreased catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), alkaline phosphatase (AKP) and acid phosphatase (ACP) activities, and glutathione (GSH) levels in mussel tissues. RES significantly decreased the MDA levels in mussel tissues. However, the MDA level increased significantly with the increase of RES treatment concentration. Furthermore, the activities of CAT, SOD GSH-PX, AKP, and ACP, and the GSH levels in gonad and gill, those of CAT and AKP, and the GSH level in adductor muscle and mantle, and those of SOD and GSH-PX in mantle were increased first and then decreased with the increase of RES concentration. In addition, 10 or 20 μmol/L RES treatment significantly slowed down the degradation of follicular and gamete in mussel gonad and the losing of gill filaments of mussels caused by starvation stress. There were no obvious pathological changes in the adductor muscle and mantle tissue of mussels in each group. These results indicate that starvation stress inhibited the antioxidant capacity of M. coruscus. 10 or 20 μmol/L RES might alleviate the lipid peroxidation and oxidative stress damage due to starvation. However, starvation stress-induced oxidative stress and antioxidant enzyme activities are tissue-specific in M. coruscus.
Key words:  Mytilus coruscus  starvation stress  oxidative stress  enzymatic activity  tissue damage
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