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引用本文:林治豪,张焕新,唐学玺,王影.环烷酸对淡水微藻生理生化特征的影响.海洋与湖沼,2023,54(1):87-97.
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环烷酸对淡水微藻生理生化特征的影响
林治豪1, 张焕新2, 唐学玺1,3, 王影1,3
1.中国海洋大学海洋生命学院 山东青岛 266003;2.山东师范大学地理与环境学院 山东济南 250014;3.青岛海洋科学与技术试点国家实验室 海洋生态与环境科学功能实验室 山东青岛 266237
摘要:
环烷酸是高酸原油的主要污染组分,对水生生态系统造成潜在威胁。在不同浓度环烷酸处理下对两种模式淡水微藻——莱茵衣藻(Chlamydomonas reinhardtii)和小球藻(Chlorella vulgaris)进行了96 h的连续培养,研究了环烷酸胁迫对两种淡水微藻种群及生理生化特征的影响,旨在为水生生态系统中环烷酸污染的生态风险预测与规避提供前瞻性的研究资料。研究结果表明,低浓度环烷酸(0.5~4mg/L)会刺激淡水微藻的种群增长,而高浓度环烷酸(8~16mg/L)会抑制淡水微藻的种群增长,造成急性毒性损伤。低浓度环烷酸处理下两种微藻叶绿素a含量上升,脂质积累,高浓度环烷酸处理下两种微藻叶绿素a含量下降而多糖含量上升,推测环烷酸剂量可能会影响淡水微藻能量存储方式。两种微藻在环烷酸胁迫下均会受到氧化损伤,造成活性氧(reactive oxygen species,ROS)含量上升,两种微藻抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)对环烷酸处理具有种属差异;高浓度环烷酸对淡水微藻的急性损伤具有时间效应,毒性随着胁迫时间的延长而逐渐增强。综上,环烷酸对淡水微藻的种群及生理过程具有明显的剂量和时间效应,研究结果可为淡水水生生态系统的环烷酸污染的风险评估提供数据支撑。
关键词:  环烷酸  淡水微藻  种群动态  生化特征  抗氧化酶活性  毒物兴奋效应
DOI:10.11693/hyhz20220400096
分类号:X55
基金项目:国家自然科学基金,41976132号,61902368号,42176204号;中央高校基本科研专项资金,201964025号;国家自然科学基金-山东省联合基金,U1606404号。
附件
EFFECT OF NAPHTHENIC ACID ON PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERISTICS OF FRESHWATER MICROALGAE
LIN Zhi-Hao1, ZHANG Huan-Xin2, TANG Xue-Xi1,3, WANG Ying1,3
1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;2.College of Geography and Environment, Shandong Normal University, Jinan 250014, China;3.Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
Abstract:
Two models of freshwater microalgae, Chlamydomonas reinhardtii and Chlorella vulgaris, were continuously cultured for 96h under different concentrations of naphthenic acids (NAs). The effects of NAs stress on the population and physiological and biochemical characteristics of two freshwater microalgae were studied. Results show that low concentrations of NAs (0.5~4 mg/L) stimulated the population growth of microalgae, while high concentrations of NAs (8~16 mg/L) inhibited the population of microalgae growth, causing acute toxic damage. Treatment of NAs could change the physiological activity of microalgae. In low-concentration NAs treatment, content of chlorophyll a in the two microalgae increased and lipids accumulated, and in the treatment with high concentration of NAs, the content of chlorophyll a in the two microalgae decreased and the content of polysaccharide increased. Both microalgae were subjected to oxidative damage under NAs stress, causing an increase in reactive oxygen species (ROS) content; and two microalgal antioxidant enzymes, i.e., superoxide dismutase (SOD) and catalase (CAT) showed species-specific differences in the response to NAs treatment. In addition, high concentrations of NAs had a time effect on the acute injury of freshwater microalgae, and the toxicity gradually increased with the increase of experimental duration. Therefore, low concentrations of NAs have stimulant effects on microalgae and high concentrations of NAs have time-dependent acute toxic effects on microalgae growth. This study provided a data-support for the risk assessment of NAs pollution in freshwater environments.
Key words:  Naphthenic acid  freshwater microalgae  population dynamics  biochemical characteristics  antioxidant enzyme activity  stimulant effect
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