| 摘要: |
| 2018年4月在浙江省舟山近海海域的渔业资源调查中采集到19尾菖鲉属鱼类标本，经鉴定发现为三色菖鲉（Sebastiscus tertius），为中国大陆近海海域的新记录种。本研究对采集到的19尾三色菖鲉标本拍摄照片并对其进行形态特征以及DNA条形码研究。三色菖鲉具有以下主要形态特征：背鳍数ⅩⅡ-12，胸鳍数18—19，腹鳍数Ⅰ-5，臀鳍数Ⅲ-5；第一鳃弓总鳃耙数6—7+13—17；前鳃盖骨有5枚硬棘，鳃盖骨后沿有2枚硬棘；眶前骨下后角有1枚硬棘；胸鳍处有一明显黑斑。测定了19尾标本的12S rRNA和COI序列，结合GenBank中所有学名为Sebastiscus tertius的同源序列进行分析，发现12S rRNA序列完全一致，可作为该物种的微型DNA条形码，而COI序列中所有个体明显分为两个分支，两者遗传距离达到0.044，表明两分支可能为不同的有效种。COI同源序列分析结果显示，GenBank中学名为Sebastiscus tertius的鱼种与褐菖鲉（Sebastiscus marmoratus）聚为一支，表明GenBank中的Sebastiscus tertius存在错误鉴定的情况。 |
| 关键词: 三色菖鲉 新记录种 形态特征 DNA条形码 微型DNA条形码 |
| DOI：10.11693/hyhz20191200275 |
| 分类号:Q178.53;S932.4 |
| 基金项目:国家重点研发计划，2019YFD0901301号；国家自然科学基金项目，41776171号 |
附件 |
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| A NEW RECORD OF SEBASTISCUS TERTIUS (SCORPAENOIDEI: SEBASTIDAE) IN CHINESE MAINLAND COASTAL WATERS IN MORPHOLOGICAL CHARACTERS AND DNA BARCODING |
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JIA Cheng-Hao1, GAO Tian-Xiang2, XU Sheng-Yong2, SONG Na1
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1.The Key Laboratory of Mariculture(Ocean University of China), Ministry of Education, Qingdao 266003, China;2.Fisheries College, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| A rockfish species Sebastiscus tertius (Barsukov and Chen, 1978) was recorded from coastal waters of Zhoushan, Zhejiang Province, China, based on 19 specimens with 167.1—254.3mm in standard length. This species was distinct from other rockfishes in the following combination of characters: dorsal-fin rays VII, 12, anal-fin rays Ⅲ, 5, pectoral-fin rays 18—19; Gill rakers on first gill arch 6—7+13—17; 5 spines in the anterior operculum and 2 spines in the posterior edge of the operculum; 1 spine in the posterior horn of the anterior orbital bone; an obvious black spot on the pectoral fin. This species is the third Sebastiscus species recorded from China. The 12S rRNA gene fragments and cytochrome oxidase I subunit (COI) gene fragments were determined and the homologous sequences of S. tertius and in GenBank were analyzed. Results showed that the sequences of 12S rRNA were all identical with the homologous sequences of S. tertius from NCBI and could be used as the DNA barcode. The result of COI suggested that all individuals could be clearly divided into two branches with a genetic distance of 0.044, indicating that the two branches might be different effective species. In addition, combined with the homologous sequences analysis of S. marmoratus in GenBank, we found that the homologous sequences of S. tertius clustered into the same branch. We speculated that the sequences labeled as S. tertius are actually the sequences of S. marmoratus. |
| Key words: Sebastiscus tertius new record species morphological characters DNA barcoding DNA mini-barcoding |
