| 摘要: |
| 岩藻黄素是一种脂溶性类胡萝卜素，由于其水溶性差、环境敏感性强，使其应用受到限制。本研究采用有机溶剂浸提、大孔树脂纯化法，从裙带菜中提取高纯度岩藻黄素。通过复凝聚反应制备乳清分离蛋白-岩藻黄素-阿拉伯胶纳米复合物。通过透射电镜观察，纳米复合物呈光滑的球形，粒径约为44nm。红外光谱则表明，岩藻黄素与乳清分离蛋白、阿拉伯树胶发生相互作用，导致联烯键、乙酰基等特征吸收峰被遮蔽。与单一岩藻黄素相比，纳米包封的岩藻黄素具有较好的抗氧化活性稳定性。抗肿瘤实验表明，纳米复合物保留了岩藻黄素抑制HT29细胞增殖的生物活性。 |
| 关键词: 裙带菜 岩藻黄素 岩藻黄素纳米复合物 抗氧化 抗肿瘤 |
| DOI：10.11693/hyhz20190500088 |
| 分类号:R284;R285 |
| 基金项目:青岛市黄岛区自主创新重大项目，2014-3-11号；海藻活性物质国家重点实验室开放基金资助项目，SKL-BASS1704号。 |
附件 |
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| PREPARATION OF FUCOXANTHIN NANOCOMPLEXS AND THEIR ANTIOXIDANT AND ANTITUMOR ACTIVITY |
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SUN Xiao-Wen1, ZHAO Hai-Long2, ZHAO Li-Li3, XU Ying1, WANG Dong-Feng1
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1.College of Food Science and Technology, Ocean University of China, Qingdao 266003, China;2.Research Vessel Center, Ocean University of China, Qingdao 266003, China;3.State Key Laboratory of Bioactive Seaweed Substances, Qingdao Brightmoon Seaweed Group Co Ltd, Qingdao 266400, China
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| Abstract: |
| Fucoxanthin, a liposoluble carotenoid, has a limited application in food industry due to poor water solubility and strong environmental sensitivity. In the paper, high-purity fucoxanthin was prepared by organic solvent extraction in macroporous resin column chromatography form Undaria pinnatifida; then, fucoxanthin loaded whey protein isolated (WPI)-gum Arabic (GA) nanocomplexes were developed using complex coacervation. The nanocomplexes presented a smooth spherical shape with a diameter of about 44nm under TEM. The Fourier Transform infrared spectroscopy show that the typical peak of allenic bond and acetyl group vanished, indicating there was interaction between fucoxanthin and WPI/GA. The fucoxanthin nanocomplexes showed a higher antioxidant activity stability than free fucoxanthin. In addition, anti-tumor assay showed that the nanocomplexes retained the biological activity of fucoxanthin to inhibit the proliferation of HT29 cells. |
| Key words: Undaria pinnatifida fucoxanthin fucoxanthin nanocomplexs anti-oxidant anti-tumor |
