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褪黑素对雨生红球藻(Haematococcus pluvialis)生理和虾青素积累的影响
岳陈陈, 丁巍, 徐军伟, 李涛, 赵鹏, 余旭亚
昆明理工大学生命科学与技术学院 昆明 650500
摘要:
本实验初步研究了不同浓度褪黑素(melatonin,MLT)对胁迫条件下雨生红球藻Haematococcus pluvialis LUGU生长、虾青素、相关生理指标及基因表达的影响。结果表明,10μmol/L MLT可显著提高藻细胞中虾青素产量(31.32mg/g),是对照组(13.27mg/g)的2.36倍;碳水化合物和蛋白含量同比提高了1.36、1.37倍。此外,虾青素合成相关基因pdsbkt的表达水平分别提高了5.79和12.16倍。研究表明,MLT可显著促进雨生红球藻中虾青素的积累,为利用微藻生产虾青素提供了一种有效的策略。
关键词:  褪黑素  雨生红球藻  虾青素  基因表达
DOI:10.11693/hyhz20180300067
分类号:
基金项目:国家自然科学基金地区科学基金项目,21766012号,21666012号。
THE EFFECTS OF MELATONIN ON THE PHYSIOLOGICAL INDEX AND THE ACCUMULATION OF ASTAXANTHIN IN HAEMATOCOCCUS PLUVIALIS
YUE Chen-Chen, DING Wei, XU Jun-Wei, LI Tao, ZHAO Peng, YU Xu-Ya
Faculty of Life Sciences and Technology, Kunming University of Science and Technology, Kunming 650500, China
Abstract:
In this study, the effects of different concentrations of melatonin (MLT) on the growth, astaxanthin content, physiological indexes and gene expression of Haematococcus pluvialis LUGU were investigated. Results showed that the astaxanthin content significantly increased in algal cells at 10μmol/L MLT, the maximum astaxanthin content was 31.32mg/g, and enhanced by 2.36 times compared with that of the control. The carbohydrate and protein contents increased by 1.36 and 1.37 fold, respectively. Furthermore, the expression levels of the astaxanthin synthesis genes pds and bkt improved by 5.79 and 12.16 times, respectively. These results indicated that MLT could significantly enhance astaxanthin accumulation in H. pluvialis. This study proposed a strategy to efficiently produce astaxanthin by using microalgae.
Key words:  melatonin  Haematococcus pluvialis  astaxanthin  gene expression
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