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| 环境因子对浒苔生长及生源硫释放的影响 |
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谭婷婷1,2, 刘春颖1,2, 刘涛3, 许高宾1, 张磊3, 邓雪1,2, 陆小兰1,2
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1.中国海洋大学海洋化学理论与工程技术教育部重点实验室 青岛 266100;2.青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 青岛 266071;3.中国海洋大学生命学院 青岛 266071
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| 摘要: |
| 近年来，我国黄海海域大规模暴发的绿潮现象对海洋生态环境和海水中的物质迁移转化产生了重要影响。而浒苔作为绿潮暴发过程中的主要藻类，是释放二甲基硫（DMS）的优势藻类，其对海水中硫酸盐吸收转化及生源硫释放发挥着重要作用。本文通过实验室培养探讨了温度、盐度及不同形态氮营养盐对浒苔生长及释放生源硫化物的影响。结果表明，在实验范围内（盐度为25—35，温度为20—25℃），盐度对浒苔生长无明显影响，但盐度增加会促进β-二甲基巯基丙酸内酯（DMSP）的合成。在温度为20℃盐度为35时，DMSP释放达到最大值。温度增加能够促进浒苔的增长，在培养第5天，25℃下浒苔湿重比20℃增加了25%左右。培养液中的DMS含量为20nmol/L左右，约是正常黄海水的4倍，DMSP的浓度更是高于正常海水的数十倍。增加无机氮浓度会促进浒苔的生长及DMS和DMSP的释放，相比之下，NH4+-N比NO3–-N更易被浒苔吸收利用，添加两种氮源组DMS和DMSP的最高含量均比空白组高60%和30%左右。DMS/DMSP的值在10%以内变化，培养过程中DMSP表观降解比AA（丙烯酸）/（AA+DMSP）总体上低于40%。 |
| 关键词: 浒苔 生长 环境因子 二甲基硫(DMS) β-二甲基巯基丙酸内酯(DMSP) |
| DOI：10.11693/hyhz20171200314 |
| 分类号:Q945 |
| 基金项目:国家自然科学基金项目，41676065号；中央高校基本科研业务费专项，201762032号；国家重点研发计划，2016YFA0601301号，2016YFC1402101号；青岛市民生科技计划，15-8-2-4-hy号。 |
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| INFLUENCE OF ENVIRONMENTAL FACTORS ON GROWTH OF ULVA PROLIFERA AND ITS BIOGENIC SULFUR RELEASE |
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TAN Ting-Ting1,2, LIU Chun-Ying1,2, LIU Tao3, XU Gao-Bin1, ZHANG Lei3, DENG Xue1,2, LU Xiao-Lan1,2
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1.Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Qingdao 266100, China;2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3.College of Marine Life Sciences, Ocean University of China, Qingdao 266071, China
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| Abstract: |
| Outbreak of green tides in recent years in the Yellow Sea caused a negative impact on the local marine environment. As the major inductive algae of the green bloom, Ulva prolifera is a responsible alga for releasing dimethylsulfide (DMS), which plays an important role in the biogenic sulfur transfer, sulphate absorption, and sulfur cycles in seawater. In a laboratory culture, we studied the effects of temperature, salinity, and nitrogen nutrient on the growth of U. prolifera and its biogenic sulfur production. In different experiment conditions (salinity:25-35, temperature:20-25℃), salinity was not the main factor affecting the growth of U. prolifera. However, with the increase of salinity, the concentration of dimethylsulfoniopropionate (DMSP) increased and maximized at the condition of 20℃ and salinity 35. The temperature increase promoted the growth of U. prolifera, and the wet weight of U. prolifera increased about 25% at 25℃ compared with 20℃ on Day 5. In addition, DMS concentration in the culture medium was about 20nmol/L, which is approximately 4 times of the control, while DMSP was even decoupled. Therefore, the addition of nitrogen nutrition could promote U. prolifera growth and the releases of DMS and other compounds. Meanwhile, NH4+ was found easier to be assimilated than NO3-. The DMS and DMSP were about 60% and 30% higher in the nitrogen nutrient-enriched groups than that of the blank. The ratio of DMS/DMSP was lower than 10% and the ratio of AA/(AA+DMSP)-the percentage of DMSP apparent degradation-was below 40%. |
| Key words: Ulva prolifera growth environmental factors dimethylsulfide (DMS) dimethylsulfoniopropionate (DMSP) |