引用本文:	朱欢,宋会银,王清华,胡愈炘,胡征宇,刘国祥.中国球状绿藻新纪录属-索囊藻属(Choricystis).海洋与湖沼,2016,47(6):1116-1125.

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*中国球状绿藻新纪录属-索囊藻属(Choricystis)*
朱欢¹, 宋会银^1,2, 王清华^1,2, 胡愈炘^1,2, 胡征宇³, 刘国祥¹
1.中国科学院水生生物研究所中国科学院藻类生物学重点实验室武汉 430072;2.中国科学院大学北京 100049;3.中国科学院水生生物研究所淡水生态与生物技术国家重点实验室武汉 430072

摘要:

在西藏那曲的错鄂和湖北武汉的紫阳湖采集到两株淡水绿色海绵。形态观察显示，两株海绵呈现绿色是由于其内共生有球状绿藻。基于形态观察和分子系统发育分析确定为该球状绿藻为索囊藻属的寄生索囊藻（Choricystis parasitica（K.Brandt）Pröschold&Darienko）。结果表明：（1）两株藻均为纺锤形或倒卵形，长1.2-2.8μm，宽0.8-1.5μm；具一个侧位片状色素体；（2）依据18S rDNA和rbcL cpDNA的进化分析显示这两株藻均位于胶球藻科（Coccomyxaceae），索囊藻属（Choricystis）；（3）基于ITS2的二级结构分析显示这两株藻与寄生索囊藻具有相同的二级结构，表明这两株内共生的球状绿藻均为寄生索囊藻。寄生索囊藻为中国新纪录种，所在属索囊藻属为中国新纪录属。本研究以形态性状和分子性状描述了采自中国的寄生索囊藻标本，为今后研究提供依据和参考。

关键词: 球状绿藻二级结构淡水海绵索囊藻属系统发育

DOI：10.11693/hyhz20160500104

分类号:

基金项目:科技部科技基础性工作专项项目，2014FY210700号。

附件

THE ENDOSYMBIOTIC GENUS CHORICYSTIS (TREBOUXIOPHYCEAE), A NEW RECORD FROM CHINA

ZHU Huan¹, SONG Hui-Yin^1,2, WANG Qing-Hua^1,2, HU Yu-Xin^1,2, HU Zheng-Yu³, LIU Guo-Xiang¹

1.Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.State key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Abstract:

Two green freshwater sponge samples were collected from Nagtsang Tso,Tibet and Ziyang Lake,Hubei.The endosymbiotic algae were observed within sponge cells of both samples.The coccoid algae were identified as Choricystis parasitica based on morphological observation and phylogenetic analysis.The main results including:(1) both strains were broadly ellipsoid,with a size about 1.2-2.8μm in length and 0.8-1.5μm in width and the chloroplast were parietal without pyrenoid;(2) the phylogenetic analysis based on 18S rDNA and rbcL cpDNA showed that the two strains were nested in the family Coccomyxaceae,the genus Choricystis;(3) The two strains shared a same ITS2 secondary structure with the type species,Choricystis parasitica.This paper first described the species Choricystis parasistica and the genus Choricysits in China,provided the morphological and molecular evidences of Chinese Choricystis specimens,and enriched the diversity of green algae in China with a useful reference about this group for further studies.

Key words: coccoid green algae ITS2 secondary structure freshwater sponge Choricystis Phylogeny