| 摘要: |
| 尽管单一逆境条件对浮游植物产生的影响已被广泛重视，然而对于多重逆境条件对藻细胞生长生理的研究仍有空白。本研究通过测定强壮前沟藻（Amphidinium carterae Hulbert）光密度（OD750）、叶绿素a含量（chl a）、光合作用效率（Fv/Fm）、碳氮比（C/N）等多个生理指标，分析其在9种环境下（常温光照、低温和低温黑暗三种物理环境，结合全营养、缺氮、缺磷三种营养状态）对多重逆境的生长和生理响应。研究结果表明，在多重逆境条件下，单一物理逆境因素（低温或黑暗）较氮限制或磷限制对藻细胞生长和生理的不利影响更为显著。营养限制并同低温环境双重作用对生物量和碳氮比产生显著性影响（P<0.01）。此外，低温和黑暗条件耦合作用下，SYTOX Green染色强度处于较低水平，chl a稳定、Fv/Fm有所升高，强壮前沟藻在逆境环境下作为群体的衰亡得以缓解。 |
| 关键词: 强壮前沟藻 低温环境 黑暗环境 氮限制 磷限制 多重逆境 |
| DOI：10.11693/hyhz20160300068 |
| 分类号: |
| 基金项目:国家基金委青年科学基金项目，41506129号；清华大学自主科研计划项目，20131089253号；深圳市科技计划基目，KQCX201533115153644号，JCYJ20150529164918736号。 |
附件 |
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| THE GROWTH AND PHYSIOLOGICAL CHANGES OF AMPHIDINIUM CARTERAE HULBERT UNDER ENVIRONMENTAL STRESSES |
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ZHANG Zhen-Ni1,2, JIANG Yue-Lu1,2, LI Man-Lu1,2, WEI Peng-Ji1,2
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1.Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China;2.School of Environment, Tsinghua University, Beijing 100084, China
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| Abstract: |
| Environmental stresses can cause the recession or death of phytoplankton in the natural aquatic environment. Though single environment stress is well documented, the coupled impacts of two or more environmental stresses on the growth and physiological changes of microalgae and the mechanisms still need further study. This study examined OD750, chl a, Fv/Fm and C/N of Amphidinium carterae Hulbert under nine different environments (normal, cold and cold dark physical conditions, combined with full-nutrient, N-deprived or P-deprived medium) to test the growth, physiological and biochemical changes of algae cells. The study shows that as the single adverse condition, coldness and darkness have more significant effects on the growth, death and physiological changes of A. cartere than N-deprived of P-deprived conditions. But when nutrients deprivation and coldness occur together, it will significantly affect the growth and cellular composition of A. carterae (P<0.01). Besides, the coupling of coldness and darkness stresses happening together may even alleviate the cell death progress in adverse environment with low SYTOX Green dyeing results, stable chl a concentration and slightly growing Fv/Fm. |
| Key words: Amphidinium carterae cold environment dark environment N-deprived medium P-deprived medium multiple environmental stresses |
