引用本文:	初双双,张云云,苏倡,王颖,陈丽萍,周君,张迪骏,司开学,苏秀榕.可口革囊星虫(Phascolosoma esculenta)重组铁蛋白富集Pb²⁺等重金属特性和功能研究.海洋与湖沼,2015,46(6):1358-1364.

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*可口革囊星虫(Phascolosoma esculenta)重组铁蛋白富集Pb²⁺等重金属特性和功能研究*
初双双¹, 张云云¹, 苏倡², 王颖¹, 陈丽萍¹, 周君¹, 张迪骏¹, 司开学¹, 苏秀榕¹
1.宁波大学海洋学院宁波 315211;2.宁波城市学院宁波 315800

摘要:

利用可口革囊星虫重组铁蛋白富集Pb²⁺、Fe³⁺、Cd²⁺、Cr³⁺、Cu²⁺、Mn²⁺、Sn²⁺和Zn²⁺等8 种重金属离子后, 通过透射显微镜和傅里叶红外光谱测定蛋白的超微结构变化和内部基团特性。以天然的马脾铁蛋白为对照, 观察重组铁蛋白与天然铁蛋白的差别。结果显示, 重组铁蛋白富集Pb²⁺ 和其它7 种重金属离子后蛋白体积大小与重金属离子种类直接相关。重组铁蛋白相对天然铁蛋白来说在富集不同种类重金属离子后, 除了非特异性酰胺特征峰和蛋白特征峰外, 还出现一些专门富集某些重金属离子的特异基团, 如:-CH₃、-CH₂-、-CH-、-NH-、-SO₃-、-COOH、-S=S-和-C-S-C 等。通过细胞实验进一步验证了铁结合蛋白能够通过富集重金属离子Pb²⁺来保护小鼠成骨前体细胞 MRC3T3-E1, 使细胞的死亡率明显降低(P<0.05).

关键词: 可口革囊星虫重组铁蛋白富集铅离子超微结构小鼠成骨前体细胞

DOI：10.11693/hyhz20150300084

分类号:

基金项目:国家自然科学基金资助项目, 40776075 号, 41176123 号; 宁波大学研究生科研创新基金项目, G14042 号。

附件

RESEARCH OF CHARACTERISTICS AND FUNCTIONS OF ENRICHMENT OF Pb²⁺ BY RECOMBINANT FERRITIN FROM PLASCOLOSOMA ESCULENTA

CHU Shuang-Shuang¹, ZHANG Yun-Yun¹, SU Chang², WANG Ying¹, CHEN Li-Ping¹, ZHOU Jun¹, ZHANG Di-Jun¹, SI Kai-Xue¹, SU Xiu-Rong¹

1.School of Marine Sciences, Ningbo University, Ningbo 315211, China;2.Ningbo City College of Vocational Technology, Ningbo 315800, China

Abstract:

The recombinant ferritin from Phascolosoma esculenta were exposed to the same concentration of different heavy metals to absorb them. The 8 kinds of heavy metals were Pb²⁺, Fe³⁺, Cd²⁺, Cr³⁺, Cu²⁺, Mn²⁺, Sn²⁺and Zn²⁺. Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) were applied to study the ultra-structure and the change of groups structure. It compared to natural horse spleen ferritin with the same processing. The results indicated that the ultra-structure of ferritin was altered after trapping different kinds of heavy metals. Recombinant ferritin groups changed after trapping different heavy metals. There were some specific groups for binding heavy metals, including-CH₃,-CH₂-,-CH-,-NH-,-SO₃-,-COOH,-S=S-,-C-S-C etc., besides some non-specific amides characteristic peaks and ferritin characteristic peaks. Further experiments verified that ferritin could protect the mouse osteoblast precursor cells MRC3T3-E1 through enrichment of Pb²⁺and other heavy metal ions, and the cell mortality was significantly lower (P<0.05).

Key words: Phascolosoma esculenta recombinant ferritin enrichment lead ion ultra-structure mouse osteoblast precursor cells