| 摘要: |
| 为了研究景天科中药植物垂盆草(Sedum sarmentosum)水提物对草鱼(Ctenopharyngodon idellus)脂肪性肝损伤治疗作用及机理, 本试验通过对连续投喂6周8.1% (鱼油5%+大豆油3.1%)高脂饲料建立的草鱼脂肪性肝损伤模型, 投喂添加1200mg/kg、300mg/kg浓度的垂盆草饲料进行6周治疗, 测定血清主要脂肪代谢相关生化指标、制作肝脏病理切片及应用半定量RT-PCR技术检测肝细胞脂肪代谢相关酶肉碱棕榈酰转移酶I(CPT-1)、过氧化物酶体增殖物激活受体-α(PPAR-α)及肿瘤坏死因子-α(TNF-α) mRNA相对表达量变化。结果表明: 连续投喂6周的高脂饲料草鱼血清生化指标发生显著变化, 组织病理学观察肝细胞发生严重的脂肪变性, 故已成功建立了草鱼脂肪性肝损伤模型。通过6周治疗, 1200mg/kg剂量组、300mg/kg剂量组均可显著降低模型动物的谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)和总胆固醇(CHO)(P<0.01);组织病理学观察显示, 1200mg/kg、300mg/kg剂量组的肝细胞脂肪变性程度与高脂组相比有一定程度的改善;同时半定量RT-PCR检测结果表明: 用药1200mg/kg、300mg/kg剂量组草鱼肝脏CPT-1 mRNA、PPAR-α mRNA相对表达量均显著高于高脂组(P<0.01), 且1200mg/kg剂量组TNF-α mRNA表达量显著低于高脂组(P<0.01)。本研究结果表明肝脏是草鱼脂肪沉淀的主要场所, 饲料中添加垂盆草水提物可对8.1%高脂饲料引起的草鱼肝脂肪性组织损伤具有治疗作用, 该治疗作用与促进肝细胞脂质代谢途径有关。 |
| 关键词: 垂盆草 脂肪性肝损伤 草鱼 |
| DOI：10.11693/hyhz20150500136 |
| 分类号: |
| 基金项目:四川省科技厅科技支撑项目,2014NZ0003号。 |
附件 |
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| THERAPEUTIC EFFECTS OF SEDUM SARMENTOSUM WATER EXTRACTION ON CTENOPHARYNGODON IDELLUS WITH FATTY LIVER INJURY |
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WU Chun-Yan1,2, WANG Kai-Yu1, REN Si-Yu1,2, WANG Jun1, MIAO Chang-Hong3
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1.Fisheries Disease Research Center, Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;2.Department of Animal Science and Technology, Chongqing Three Gorges Vocational Colleges, Chongqing 404155, China;3.Sichuan Animal Health Inspection, Chengdu 610000, China
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| Abstract: |
| In order to gain more knowledge about the therapeutic effects of Sedum sarmentosum on Ctenopharyngodon idellus with fatty liver injury.The C.idellus were fed with a 8.1 percent of high-fat-diet within six weeks to build the fatty liver model, which contained with 5 percent of fish oil and 3.1 percent of soy bean oil.And then, the C.idellus were treated with two doses of the S.sarmentosum water extraction, 1200 mg/kg and 300 mg/kg respectively.The fatty metabolism related biochemical indexes, the pathological section of the liver and pancreas, the trend of CPT-1, PPAR-α and TNF-α were detected.The results showed that the biochemical indexes were significantly changed, and the histopathological of liver cells have serious adipose degeneration.Within the six weeks of treatment with 1200mg/kg and 300mg/kg , ALT, AST, TG and CHO had remarkable decreased.Histopathological observation showed that both groups had improvement on the level of adipose degeneration of liver cell.Meanwhile, RT-PCR results showed that fish fed with 1200mg/kg and 300mg/kg had significantly increased in CPT-1 mRNA, PPAR-α, mRNA (P<0.01).And, TNF-α mRNA was significantly decreased in the fish fed with 1200mg/kg than that of other three groups (P<0.01).The results also pointed out that the S.sarmentosum could play an important role in the treatment of liver fatty tissue injury, and this treatment have a relation with the lipid metabolic pathways of liver cells. |
| Key words: Sedum sarmentosum fatty liver injury Ctenopharyngodon idellus |
