引用本文:	张娜,黄雯,许飞,李莉,张国范,郭希明.长牡蛎(Crassostrea gigas)两个Dmrt家族基因的时空表达.海洋与湖沼,2015,46(3):717-724.

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*长牡蛎(Crassostrea gigas)两个Dmrt家族基因的时空表达*
张娜^1,2, 黄雯^1,2, 许飞¹, 李莉¹, 张国范¹, 郭希明³
1.海洋生态养殖技术国家地方联合工程实验室中国科学院海洋研究所青岛 266071;2.中国科学院大学北京 100049;3.Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris, New Jersey USA 08349

摘要:

本文以长牡蛎27 个幼虫发育阶段个体以及成体的5 个组织织作为实验材料, 采用实时荧光定量PCR 技术对其Dmrt 家族中的2 个基因(CgDsx 和CgDmrtA2)的表达模式以及在性别决定中的作用进行了研究。结果表明, 长牡蛎CgDsx 基因在胚胎发育初期有大量表达, 从囊胚期到担轮幼虫初期表达量最高, 之后表达量开始降低, 在D 形幼虫后一直维持在极低的表达水平, 此后仅在成体的雄性性腺中具有高度表达。由此可见,CgDsx 可能也对早期胚胎发育起一定调控作用, 同时参与了性别决定。CgDmrtA2 在长牡蛎所有组织中均有表达, 各组织间表达差异不显著, 在D 形幼虫至壳顶后期表达量较高, 说明它参与了胚胎中后期的发育过程, 可能与神经的形成相关, 但是其具体功能还需要进一步研究。

关键词: 长牡蛎 Dmrt 家族幼虫发育组织表达差异性别决定

DOI：10.11693/hyhz20140900249

分类号:

基金项目:国家重点基础研究发展计划(973 项目)资助, 2010CB126401 号; 泰山学者计划。

附件

EXPRESSION OF TWO Dmrt FAMILY GENES IN THE PACIFIC OYSTER CRASSOSTREA GIGAS

ZHANG Na^1,2, HUANG Wen^1,2, XU Fei¹, LI Li¹, ZHANG Guo-Fan¹, GUO Xi-Ming³

1.National & Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Rutgers University, New Jersey, USA, 08349

Abstract:

We detected the expression profiles of two Dmrt family genes in the larval of different stages in adult tissues, and their possible roles were also conducted in the Pacific oyster Crassostrea gigas. Quantitative real-time PCR was used to determine mRNA expression at 27 larval stages in 5 adult tissues. The result shows that the CgDsx gene was expressed during early embryonic development and peaked between blastula and early trochophore stages, then decreased after D-stage. Our findings indicate that CgDsx may play a role in regulating embryonic development in addition to its role in sex determination. CgDmrtA2 was expressed in all adult tissues with no significant difference; it was highly expressed in swimming larvae between D- and late umbo stages, suggesting that CgDmrtA2 may be involved in larval development, perhaps related to nerve development. More studies are called in the future on exact function of the genes.

Key words: Crassostrea gigas Dmrt family genes larval development tissue specific expression sex determination