| 摘要: |
| 选用540 尾初重为(6.38±0.26)g 的欧洲鳗鲡(Anguilla anguilla), 随机分为6 个处理(每个处理3 个重复, 每个重复30 尾鱼), 分别投喂添加了0.05% (Diet 1)、0.10% (Diet 2)、0.15% (Diet 3)、0.20% (Diet 4)、0.25% (Diet 5)的复合酶制剂饲料, 另增设基础饲料为对照组(Diet 0), 研究复合酶制剂对欧鳗生长性能、消化酶活性及非特异性免疫的影响。6 周投喂结果表明: 随着复合酶制剂添加量的递增欧鳗增重率和特定生长率呈先增后降趋势, 其中Diet 3 组增重率和特定生长率分别比对照组提高了34.22%和26.36%(P<0.05), 肝体指数及肥满度各组之间差异不显著(P>0.05); 复合酶制剂降低了鱼体粗灰分含量, 对鱼体含水量、粗脂肪及粗蛋白含量影响较小, 均无显著差异(P>0.05); 相比对照组各添加组鳗鱼肠道胰蛋白酶活性显著提高(P<0.05), 淀粉酶活性则随酶制剂浓度的增高呈先升后降趋势, 脂肪酶活性受酶制剂影响较小, 组间无显著差异(P>0.05); 各添加组鳗鱼血清中超氧化物歧化酶(SOD)、溶菌酶(LZM)、碱性磷酸酶(AKP)活性相比对照组均有不同程度的提高, 所有添加组鳗鱼血清总蛋白含量(TP)均显著高于对照组, Diet 3 组(0.15%)相比对照提高了38.86%。综上所述,饲料中添加适量的复合酶制剂具有促进鳗鲡生长、提高肠道消化酶活性和非特异性免疫的功能, 本试验条件下酶制剂最适添加浓度为0.15%。 |
| 关键词: 欧洲鳗鲡 复合酶制剂 生长性能 体成分 消化酶 免疫酶 |
| DOI：10.11693/hyhz20141000278 |
| 分类号: |
| 基金项目:厦门市科技项目:鳗鲡环保型抗病促长添加剂的研制(3502Z20123026)。 |
附件 |
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| EFFECTS OF MULTI-ENZYME ON GROWTH PERFORMANCE, ACTIVITIES OF DIGESTIVE ENZYMES AND IMMUNE RESPONSE OF ANGUILLA ANGUILLA |
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LU Jing1,2, LI Zhong-Bao1,2, CHEN Qiang1,2, LI Wen-Jing1,2, HUANG Yong-Chun1,2
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1.Fisheries College, Jimei University, Xiamen 361021, China;2.Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-environment, Xiamen 361021, China
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| Abstract: |
| A feeding trial was conducted to study the effects of multi-enzyme on growth performance, digestibility, and immune response of Anguilla anguilla. Five hundred and forty fish were randomly allotted to six groups with triplication and fed diets containing multi-enzyme at a rate of 0.05% (Diet 1), 0.10% (Diet 2), 0.15% (Diet 3), 0.20% (Diet 4), 0.25% (Diet 5), and the control (Diet 0). After feeding for 6 weeks, the weight gain rate (WGR) and specific growth rate (SGR) increased and then decreased with increasing multi-enzyme in diets. In addition, the WGR and SGR of Diet 3 were greater than those of the control by 34.22% and 26.36%. However, no significant difference was found among all the treatments in hepatosomatic index or condition factor (P>0.05). The feed added multi-enzyme decreased the ash content, but no significant differences in body moisture, crude lipid content, and crude protein from those of the control (P>0.05). The apparent activities of intestinal trypsin were significantly higher in Diet 1—Diet 5 (P<0.05); amylase activities increased and then decreased with the multi-enzyme concentration; no significant differences in lipase in the fish fed with different diets (P>0.05). The activities of serum superoxide dismutase, lysozyme; alkaline phosphatase of multi-enzyme added groups increased significantly from those of the control (P<0.05); and the concentration of serum total protein of Diet 3 (0.15%) was higher than the control by 38.86% (P<0.05). Therefore, the feed added with 0.15% multi-enzyme can promote the growth performance, digestibility, and immune response of A. anguilla. |
| Key words: Anguilla anguilla multi-enzyme growth performance activities of digestive enzymes immune response |
