| 摘要: |
| 本试验旨在研究饲料维生素C对美国红鱼(Sciaenops ocellatus)组织中的NO含量、NOS活力和NOS基因表达的影响。采用单因子试验设计, 将360尾平均体重为(147.28±7.69)g的美国红鱼随机分为6个处理, 每个处理设3个重复, 每个重复20尾, 分别饲喂6种不同维生素C水平(0、49.3、98.9、197.5、396.4和1989.8 mg/kg)的等氮等能饲料, 进行为期8周的生长试验。结果表明: 头肾、肝、肠、胸腺NO含量随着饲料维生素C含量增加有显著增加; 脾中各组NO含量无显著性差异; 脑中NO含量C0组与其它各组相比有显著性差异。头肾、肝、胸腺NOS活力随着饲料维生素C含量增加有显著增加; 脾和肠中各组NOS活力无显著性差异; 脑中NOS活力C396.4组与其它各组相比有显著性差异。脑中各组nNOS基因mRNA的表达含量无显著性差异; 在饲料维生素C含量达到1989.8 mg/kg时, 其它各组织内的nNOS基因mRNA的表达量最高。综上所述, 饲料中添加适当维生素C对美国红鱼组织中的NO含量、NOS活力和nNOS基因mRNA的表达有显著影响。 |
| 关键词: 美国红鱼 维生素C 一氧化氮 免疫 |
| DOI：10.11693/hyhz20140700193 |
| 分类号:S917；Q50 |
| 基金项目:广东省高等学校高层次人才项目, 粤财字[2013]246 号; 广东省海洋生物技术重点实验室开发基金资助项目, GPKLMB201202 号; 国家自然科学基金项目, 31201978 号; 惠州市科技计划项目, 2011B040010003 号; 惠州学院自然科学研究重点项目,2012ZD07 号 |
附件 |
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| EFFECTS OF DIETARY VITAMIN C ON NITRIC OXIDE, NITRIC OXIDE SYNTHASE ACTIVITY AND NITRIC OXIDE SYNTHASE GENE EXPRESSION IN TISSUE OF RED DRUM SCIAENOPS OCELLATUS |
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Zhou Libin,Bai Ru,Ma Xilan,Wang Shuqi and Zhang Haifa
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College of Life Science, South China Normal University, Guangzhou,,Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University，Shantou 515063,Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University，Shantou 515063,Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University，Shantou 515063,Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University，Shantou 515063
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| Abstract: |
| We conducted an experiment to study the effects of dietary vitamin C on nitric oxide, NOS activity and nNOS mRNA expression in tissues of red drum Sciaenops ocellatus. Six iso-nitrogenous and iso-energetic diets were formulated to contain graded level of vitamin C (0, 49.3, 98.9, 198.7, 398.6, and 1987.4 mg/kg diet, respectively ) and fed to 18 floating net cages (1.5?1.0?1.0 m) each containing 20 fish (initial weight: 147g) twice daily (07:30, 16:30) for 8 weeks. Results indicate that after 8-week feeding trial, the levels of nitric oxide (NO) content and nitric oxide synthase (NOS) activity in head, kidney, liver, and thymus were boosted with increasing dietary vitamin C from 0 to 1987.4 mg/kg (P<0.05), but in spleen and intestines, no significant difference was found among dietary treatments. The NOS activity reached a peak value in brain fed at 398.6 mg/kg of dietary vitamin C. No significant difference in nNOS mRNA expression in brain was found among dietary treatments; the level of nNOS mRNA expression was found maximal in all tissues except for brain when fed 1987.4 mg/kg of dietary vitamin C. Therefore, adding dietary vitamin A in feed a proper amount could increase remarkably the NO content, NOS activity, and nNOS mRNA expression of red drum. |
| Key words: red drum Sciaenops ocellatus vitamin C nitric oxide immunity |
