引用本文:	王稷,李晔,瞿洋,沈灵灵,孙静,全晶晶.地衣芽孢杆菌(Bacillus licheniformis)对镉胁迫下泥蚶(Tegillarca granosa)的抗氧化酶活性和免疫相关基因表达的影响.海洋与湖沼,2014,45(5):1058-1063.

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*地衣芽孢杆菌(Bacillus licheniformis)对镉胁迫下泥蚶(Tegillarca granosa)的抗氧化酶活性和免疫相关基因表达的影响*
王稷,李晔①,瞿洋,沈灵灵,孙静,全晶晶
宁波大学海洋学院宁波 315211,宁波大学海洋学院宁波 315211,宁波大学海洋学院宁波 315211,宁波大学海洋学院宁波 315211,宁波大学海洋学院宁波 315211,宁波大学海洋学院宁波 315211

摘要:

本文研究一株地衣芽孢杆菌(Bacillus licheniformis)对提高泥蚶耐受镉(Cd²⁺)胁迫的作用, 实验分析了不同浓度地衣芽孢杆菌对Cd²⁺胁迫下泥蚶(Tegillarca granosa)的存活率及组织中Cd残留量的影响; 同时比较了地衣芽孢杆菌作用前后, 泥蚶体内两类抗氧化酶, 超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性, 进一步通过Real-time PCR分析了三类免疫相关基因金属硫蛋白(MT)、小热休克蛋白分子(sHSP)和铁蛋白(ferritin)的表达情况。研究结果显示, 地衣芽孢杆菌能降低泥蚶组织中Cd残留量, 提高其存活率。并能有效促进泥蚶体内SOD和GSH-Px酶活性的增加, 同时降低MT、sHSP和ferritin在mRNA水平上的表达。数据表明该地衣芽胞杆菌可通过调节泥蚶的免疫系统, 起到降低Cd²⁺对泥蚶毒害的作用。

关键词: 地衣芽孢杆菌泥蚶镉残留量存活率抗氧化酶免疫基因

DOI：10.11693/hyhz20140600187

分类号:

基金项目:浙江省海水养殖科技创新团队资助项目, 2010R50025 号

附件

INTAKE OF BACILLUS LICHENIFORMIS MEDIATES ANTIOXIDANT ENZYME ACTIVITIES AND GENE EXPRESSION IN CADMIUM-STRESSED TEGILLARCA GRANOSA

Wang Ji,Li Ye,Qu Yang,Shen LingLing,Sun Jing and Quan Jingjjing

Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University,,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University

Abstract:

To assess the ability of Bacillus licheniformis in removing Cd from aquatic animals, Tegillarca granosa was used to test the effect of B. licheniformis intake on Cd removal, and the impacts on the survival, the activities of two antioxidant enzymes, and the expression levels of three genes in T. granosa that were co-administrated in Cd. Results show that B. licheniformis intake effectively reduced the Cd contents in visceral mass of T. granosa and increased the survival rates. Meanwhile, the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were significantly increased. However, the expression levels of metallothionein (MT), small heat shock protein (sHSP) and ferritin were all down-regulated in T. granosa fed B. licheniformis compared to the control. Therefore, B. licheniformis could protect T. granosa potentially and effectively from stress by reducing Cd toxicity and modulating the immune system of the host.

Key words: Bacillus licheniformis Tegillarca granosa cadmium chloride survival rate antioxidant enzymes immune-related genes