| 摘要: |
| 采用分子生物学方法, 对分离的米氏凯伦藻4株藻际异养细菌进行分子鉴定和功能分析。结果表明, 经16S rRNA序列分析, 4株藻际异养细菌分别隶属于Formosa、Erythrobacter、Shewanella和Marinobacter四个属, P1菌株与Formosa 的16S rRNA核苷酸序列的同源性为95%, P2、P3和P4菌株与Erythrobacter、Shewanella和Marinobacter 的同源性在99%以上。通过4株异养细菌及其同源性大的24株细菌构建进化树得出, 28株细菌在系统发育树中主要分成4个分支, 与同源性分析相符。 |
| 关键词: 米氏凯伦藻 藻际异养细菌 16S rRNA 分子鉴定 |
| DOI：10.11693/hyhz20140600174 |
| 分类号: |
| 基金项目:浙江省科技厅项目, 2014C32074 号; 浙江省自然科学基金项目, LY12C03001 号; 宁波大学学科项目, XKL11D2100 号 |
附件 |
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| MOLECULAR IDENTIFICATION OF FOUR STRAINS OF HETEROTROPHIC BACTERIA ISOLATED FROM KARENIA MIKIMOTOI |
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GONG Shi-yan,TU Yan-ping and XIE Zhi-hao
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KeySLaboratorySofSAppliedSMarineSBiotechnology,SMinistrySofSEducation,SNingboSUniversity,SNingbo,KeySLaboratorySofSAppliedSMarineSBiotechnology,SMinistrySofSEducation,SNingboSUniversity,SNingbo,KeySLaboratorySofSAppliedSMarineSBiotechnology,SMinistrySofSEducation,SNingboSUniversity,SNingbo
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| Abstract: |
| Molecular identification and functional analysis on heterotrophic bacteria from Kareoia mikimotoi were carried out. Four cultivable strains, ie., P1 to P4 that selected from these isolates were identified and classified based on analysis of 16S rRNA sequence. The results show that they belong to four genera including, Erythrobacter, Shewanella, and Marinobacter. Similarity search in 16S rRNA sequence indicated that Strain P1 could be identified Formosa to genus level, and other three strains P2 to P4 to species level for sharing more than 99% sequence homology with some strains of Erythrobacter, Shewanella, and Marinobacter, respectively. Phylogenetic tree between the four strains and their 24 homologies could be divided into four branches, which is consistent with homology analysis. |
| Key words: Karenia mikimotoi heterotrophic bacteria 16S rRNA molecular identification |
