| 引用本文: | 王中华,徐茂琴,谢 利,张迪骏,周 君,何伟娜,陈丽萍,童茜茜,张春丹,苏秀榕.宁波沿海陆源排污口拟杆菌(Bacteroidetes)分布的特点.海洋与湖沼,2014,45(5):1030-1036. |
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| 宁波沿海陆源排污口拟杆菌(Bacteroidetes)分布的特点 |
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徐茂琴,张迪骏,周君,王中华,何伟娜,陈丽萍,童茜茜,张春丹
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宁波城市职业技术学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波,宁波大学海洋学院 宁波
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| 摘要: |
| 采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析, 得到2011年3月、5月、8月、10月份各排污口总体微生物以及拟杆菌纲的分布情况。通过对454测序结果分析, 共成功鉴定24个门, 主要包括变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和后壁菌门(Firmicutes)等。其中拟杆菌门的变化趋势与梭杆菌门(Fusobacteria)相似, 均为随月份的增加而递增的趋势。在拟杆菌门下, 共鉴定出3个纲3个目9个科27个属, 4个采样月份大部分站点的优势属为黄杆菌纲(Flavobacteria), 其次为拟杆菌纲(Bacteroidetes)、鞘脂杆菌纲(Sphingobacteria), 通过聚类分析发现, 拟杆菌门分布规律主要与排污口类型和季节有一定的关系。通过454测序检测到了极难培养的寡营养细菌鞘脂杆菌, 也说明了454测序较传统微生物生态学研究方法的优势, 同时针对普雷沃菌属(Prevotella sp.)及黄杆菌属(Flavobacterium sp.)两种具有代表性的属的分布情况进行了分析。 |
| 关键词: 陆源排污口 拟杆菌 黄杆菌 鞘脂杆菌 454焦磷酸测序 |
| DOI：10.11693/hyhz20140600165 |
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| 基金项目:海洋公益性行业专项经费资助项目, 201105007 号; 浙江省重大科技攻关项目, 2006C13089 号; 宁波市科技局资助项目,2008C50027 号 |
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| DISTRIBUTION OF BACTEROIDETES IN NINGBO COASTAL SEWAGE OUTLETS |
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Xu Maoqin,Zhang Dijun,Zhou Jun,WANG zhonghua,He Weina,Chen Liping,Tong Qianqian and Zhang Chundan
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Ningbo City College of Vocational Technology, Ningbo 315100, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China,School of Marine Science, Ningbo University, Ningbo 315211, China
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| Abstract: |
| Water samples were collected in 20 sites at 10 sewage outlets in Ningbo City, Zhejiang during March, May, August, and October in 2011, and analyzed using high-throughput 454 pyrophosphate sequencing to determine the distribution of all the microbial and Bacteroidetes. Among the results, 24 phyla were identified successfully, including Proteobacteria, Bacteroidetes, Cyanobacteria and Firmicutes. The variation tendency of Bacteroidetes and Fusobacteria was similar that increased with time. In Bacteroidetes, 27 genera 9 families and 3 classes were identified. The results show that Flavobacteria was the dominant bacteria in the four months, followed by Bacteroidetes and Sphingobacteria. The type of sewage outlets and seasons affected the distribution of Bacteroidetes shown by clustering analysis. Sphingobacteria that is very difficult to culture was detected by high-throughput pyrophosphate sequencing technology (by 454 Life Science), indicating the advantage of the technology over traditional microbial ecology. Meanwhile, distributions of both Prevotella sp. and Flavobacterium sp. reprehensive of Bacteroidetes were analyzed. |
| Key words: sewage outlets Bacteroidete Flavobacteria Sphingobacteria the 454 pyrophosphate sequencing |
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