| 摘要: |
| 分别在饲料中添加0%、1%和3%聚?-羟基丁酸酯(PHB), 研究PHB对中华绒螯蟹(Eriocheir sinensis)幼蟹生长和肝胰腺酶活力的影响。将初始体重为0.8—1.0g的幼蟹(每组60只)在循环水养殖系统单独培养60d后, 对幼蟹蜕皮间隔、个体增重和肝胰腺酶活力等指标进行测定。结果表明, 随饲料中PHB添加量的增加, 幼蟹个体体重增加, 蜕皮间隔缩短, 3% PHB添加组显著高于未添加PHB的对照组(P<0.05)。肝胰腺中总超氧化物歧化酶(T-SOD)活力下降, 碱性磷酸酯酶(ALP)活力增加, 酸性磷酸酯酶(ACP)活力相对稳定, 谷草转氨酶(GOT)和谷丙转氨酶含量(GPT)活力总体较低且显著下降(P<0.05)。PHB对中华绒螯蟹幼蟹生长发育的促进作用, 除了与?-羟基丁酸提供能量物质有关外, 还与非特异免疫防御能力的提高和肝胰腺细胞良好的生理状态密切相关。 |
| 关键词: 聚-羟基丁酸酯 中华绒螯蟹 生长 肝胰腺 酶活力 |
| DOI：10.11693/hyhz201305031031 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 31172427号。 |
附件 |
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| EFFECT OF POLY--HYDROXYBUTYRATE ON GROWTH AND HEPATOPANCREATIC ENZYME ACTIVITIES OF ERIOCHEIR SINENSIS JUVENILES |
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LIU Yu1, SUI Li-Ying1, DENG Yuan-Gao1, CHANG Guo-Liang2, ZHANG Wen-Liang3
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1.Tianjin University of Science & Technology, Tianjin Key Laboratory of Marine Resources & Chemistry;2.Huaiyin Normal University, Jiangsu Engineering Laboratory for Characteristic Aquatic Species Breeding;3.School of Environmental Science and Engineering, Tianjin University,Department of Preparing of
Tianjin Bohai Oceanic Monitoring & Surveillance Management Base, Tianjin Oceanic Administration
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| Abstract: |
| Poly-?-hydroxybutyrate (PHB) is a bacterial storage compound, and can be partially degraded into water-soluble ?-hydroxybutyric acid in gastrointestinal tract of aquatic animals. Apart from being an energy source, PHB is currently considered an alternative bio-control compound (prebiotics) due to its antibacterial activity. We investigated the effect of PHB on growth and hepatopancreatic enzyme activity of Chinese mitten crab Eriocheir sinensis juveniles. Juvenile crabs in initial body weight (BW) of 0.8—1.0g were reared separately in a recirculation system for 60 days. Crabs were fed dry feeds supplemented with 0 (control), 1%, and 3% PHB at a daily ration of 10% BW. Each treatment contained 60 juveniles. The results show that the molting frequency and BW gain of the crabs were improved with increasing PHB supplementation in feed. Supplementation of 3% PHB resulted in a significantly shorter molting interval and a better BW gain than control’s (P<0.05). In hepatopancrease, PHB supplementation declined the total superoxide dismutase activity and elevated alkaline phosphatase (ALP) activity, whilst acid phosphatase (ACP) activity remained at constant level. On the other hand, both glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were kept in relative low levels but decreased significantly with increasing PHB (P<0.05). We concluded that as an energy source for promoting the growth of E. sinensis juveniles, PHB could also improve non-specific immunity for good physiological status of the hepatopancrease. |
| Key words: Poly--hydroxybutyrate Eriocheir sinensis growth hepatopancreatic enzyme activity |
