| 摘要: |
| 采用DNA条形码(DNA barcoding)技术, 基于COI基因序列鉴定牡蛎种类, 研究了牡蛎科(Ostreidae)牡蛎在广西沿海的分布。本次研究从广西沿海16个采样地点的潮间带共采集550个野生牡蛎样本, 随机抽取了367个牡蛎样本进行基因组DNA的提取和线粒体COI基因的鉴定, 共检测出4属7种牡蛎。巨牡蛎属有4种, 分别为熊本牡蛎(Crassostrea sikamea)108个, 葡萄牙牡蛎(Crassostrea gigas angulata)43个, 香港巨牡蛎(Crassostrea hongkongensis)15个, 近江牡蛎(Crassostrea ariakensis)18个, 囊牡蛎属(Saccostrea)的多刺牡蛎(Saccostrea echinata)61个, 爪蛎属(Talonostrea)的猫爪牡蛎(Talonostrea talonata)112个, 齿牡蛎属(Dendostea)的齿缘牡蛎(Dendostea crenulifera)10个。结果表明, 广西沿海牡蛎物种丰富, 至少7种牡蛎分布于潮间带, 其中优势种为猫爪牡蛎和熊本牡蛎, 在广西沿海各采样点均有分布。多刺牡蛎和葡萄牙牡蛎次之, 香港巨牡蛎、近江牡蛎、齿缘牡蛎数量较少, 分布较集中。 |
| 关键词: 广西 牡蛎科 COI 分子鉴定 分布 |
| DOI：10.11693/hyhz201305029029 |
| 分类号: |
| 基金项目:国家环境保护部项目, 物种10-二-14号; 海洋公益性行业科研专项经费项目, 200805069号; 中国科学院海洋研究所“一三五”生物多样性项目, 2012IO060101号; 国家自然科学基金项目, 40406032号, 40876084号, 31000948号; 中国科学院战略性先导科技专项, XDA1102030304号。 |
附件 |
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| CLASSIFICATION AND DISTRIBUTION OF OYSTERS OFF COASTAL GUANGXI, CHINA |
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LI Cui1, WANG Hai-Yan2, LIU Chun-Fang1, LI Yan-Wei2, GUO Xi-Ming3
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1.Institute of Oceanology, Chinese Academy of Sciences,University of Chinese Academy of Sciences;2.Institute of Oceanology, Chinese Academy of Sciences;3.Haskin Shellfish Research Laboratory, Institute of Marine and Coastal Sciences, Rutgers University, Port Norris
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| Abstract: |
| We used COI barcodes to recognize the oysters from the coastal area in Guangxi, China. A total of 550 oysters were collected from 16 sites along coast, of which 367 oysters were specified in mitochondrial COI gene-based analysis. At least 7 oyster species were recognized in this study, of which 4 were from genus Crassostrea in Family Ostreidae: C. sikamea (186 samples), C. gigas angulata (43 samples), C. hongkongensis (15 samples), and C. ariakensis (18 samples); and 3 belong to other genera, i.e., Saccostrea echinata (61 samples), Talonostrea talonata (112 samples), and Dendostea crenulifera (10 samples). The dominant ones were T. talonata and C. sikamea. |
| Key words: Guangxi Ostreidae COI molecular identification distribution |
