| 摘要: |
| 本实验室采用人工诱变技术获得一个坛紫菜耐低盐品系(YZ-7), 为了验证该品系的耐低盐性状能否遗传, 本文对其壳孢子和F1叶状体的低盐耐受性分别进行了测试, 并以坛紫菜野生品系(WT)作为对照, 结果表明: 在不同盐度下培养2 周的YZ-7和WT的壳孢子, 在26 盐度组存活率无显著差异; 在15 盐度组, YZ-7的壳孢子存活率和假根发生率分别比WT提高了34%和35%; 在8 和5盐度组, YZ-7的壳孢子存活率、分裂率及假根发生率分别比WT提高了65%、32%和62%, 以及137%、80%和154%; 在3 盐度组, YZ-7的壳孢子存活率和分裂率分别比WT提高了458%和171%, 其中约44.9%的YZ-7壳孢子萌发体能形成假根, 而WT的壳孢子萌发体基本上不长假根。在26 盐度中培养35d 后的F1叶状体再分别在26、15、8、5 和3 盐度下培养30d, YZ-7叶状体的特定生长率分别为WT的1.7、1.9、1.5、1.6 和1.6倍, 绝对生长率分别是WT的3.4、4.1、1.8、2.0 和1.6 倍。此外, YZ-7叶状体的叶绿素荧光活性(PSⅡ最大光量子产量、实际光量子产量)及三种主要光合色素(叶绿素a、藻红蛋白和藻蓝蛋白)的含量也明显高于WT。上述结果证实, 坛紫菜选育品系YZ-7是一个耐低盐且生长快的优良品系, 其优良性状可稳定传递给下一代, 有望被培育成适宜栽培的新品种。 |
| 关键词: 坛紫菜 新品系 耐低盐 壳孢子 F1叶状体 |
| DOI：10.11693/hyhz20130400022 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863 计划), 2012AA10A411 号; 国家自然科学基金项目, 31072208 号; 农业部公益性专项,200903030 号; 国家海洋局公益专项, 201105008 号, 201105023 号; 国家农业科技成果转化资金项目, 2011GB2C000005 号; 上海市科委重点科技攻关项目, 10391901100 号; 上海高校水产学一流学科建设项目 |
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| SELECTION AND CHARACTERIZATION OF A LOW-SALINITY TOLERANT STRAIN IN PYROPIA HAITANENSIS (BANGIALES, RHODOPHYTA) |
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TAN Ying-Hua,HUANG Lin-Bin and YAN Xing-Hong
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College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306,College of Fisheries and Life Science,Shanghai Ocean University,Shanghai,201306
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| Abstract: |
| A low-salinity tolerant strain (YZ-7) was selected by artificial mutagenesis in our laboratory. The tolerance of YZ-7 conchospores and F1 gametophytic blades to low-salinities was studied against the wild-type (WT) as the control in salinities of 26, 15, 8, 5, and 3 for 2 weeks. The results show no significant difference in survival rate between YZ-7 and WT at normal salinity 26. In the 15 salinity group, the survival rates and the rhizoid germination rates of YZ-7 conchospores germlings were 34% and 35% higher than those of control, respectively. In 8 and 5 salinity groups, the survival rates and division rates of conchospores, and the rhizoid germination rates were 65% and 137%, 32% and 80%, 62% and 154% higher than those of the control, respectively. In the group of lowest salinity at 3, the survival rates and division rates of YZ-7 conchospores were 458% and 171% higher than those of control, respectively, and 44.9% of YZ-7 but zero WT conchospore germlings had rhizoid germinated. In addition, we took YZ-7 blades that cultured in normal salinity 26 for 35 d and then transferred them into different salinities for another 30 days in the same salinity spectra mentioned above. The results show that the absolute growth rates of YZ-7 blades were 6.85, 8.24, 4.15, 5.80, and 2.88 times higher; specific growth rates were 2.57, 2.63, 2.79, 4.05, and 3.90 higher than those of WT, respectively to the salinities. In chlorophyll fluorescence activities (Fv/fm and Yield) and contents of three main photosynthetic pigments (chl a, PE, and PC), those of YZ-7 blades were significantly higher than those of WT. Therefore, YZ-7 were proved as a low-salinity-tolerant fast-growing strain whose traits could be passed onto the next generation, and be a potential new cultivar. |
| Key words: Pyropia haitanensis new strain low-salinity tolerant F1 gametophytic blade conchospore |
