| 摘要: |
| 本研究采用快速瑞氏-吉姆萨染色法观察鳗弧菌(Vibrio anguillarum)刺激后魁蚶(Scapharca broughtonii)几种血细胞的形态变化, 研究不同种类血细胞的免疫功能。魁蚶血淋巴液中红细胞、白细胞和血栓细胞经免疫刺激后均发生形态改变, 属于免疫防御的应激反应。经鳗弧菌免疫刺激后, 红细胞无丝分裂现象增多, 并参与病原菌的吞噬作用; 白细胞变形运动、吞噬和融合现象普遍, 细胞表面具有粘性, 参与血栓的形成; 血栓细胞主要表现出粘连和凝血的特性, 偶有吞噬病原菌的现象;研究中检测了2 种常规抗凝剂的抗凝效果, 结果表明, 2 种抗凝剂分别改变了血栓细胞和白细胞的表面粘连特性, 从而阻止它们参与凝血, 因而都具有抗凝效果。本研究结果表明, 魁蚶血细胞分工明确,能共同防御病原菌的入侵, 细胞免疫在魁蚶血淋巴液抗菌免疫中起极其重要的作用, 血细胞的形态变化和免疫功能分析可为后期研究魁蚶抗菌机制和筛选抗凝剂奠定基础。 |
| 关键词: 魁蚶 血细胞 鳗弧菌 抗凝剂 |
| DOI：10.11693/hyhz20130300009 |
| 分类号: |
| 基金项目:山东省科技发展计划项目, 2010GHY10513 号; 浙江海洋学院“海洋渔业科学与技术”重中之重学科开放课题, 20110211 号;国家科技基础条件平台项目, 2007DKA30470 号; 黄海所基本科研业务费项目, 20603022012003 号 |
附件 |
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| EFFECT OF VIBRIO ANGUILLARUM ON MORPHOLOGY AND IMMUNOLOGICAL FUNCTION OF BLOOD CELLS IN SCAPHARCA BROUGHTONII |
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Zhou Liqing
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Yellow Sea Fisheries Institute, Chinese Academy of Fishery Sciences
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| Abstract: |
| We studied morphological change of blood cells in hemolymph of Scapharca broughtonii after immunostimulation with pathogenic Vibrio anguillarum observed under oil immersion lens of Leica microscope in two regular anticoagulants. Morphological change of red blood cells, white blood cells, and thrombus cells were common in hemolymph due possibly to stress response of immune defense. Red cells often showed amitosis and split, and were often involved in phagocytosis. Deformation, phagocytosis, and fusion were common for white cells, and white cells were involved in the clots formation for their viscous mobility surface. Thrombocytes had adhesion and coagulation properties, and occasionally, they were found bacteria adhesion and phagocytosis. In addition, we tested the anticoagulation effect by the two anticoagulants, showing that the two anticoagulants could prevent white cells or thrombocytes from coagulation by changing their cell-surface characteristics. Therefore, they could be used as reliable anticoagulants. The immunological function played by each type of blood cells is very clear in cellular immunity. The morphological changes and immunological function enriched our understanding on blood immunological mechanism of Scapharca broughtonii, and
may help develop anticoagulants. |
| Key words: Scapharca broughtonii Vibrio anguillarum blood cells anticoagulant |
