引用本文:	张滔,刘相全,孙振兴,乔洪金,孙国华,刘丽娟,宋向军.大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析.海洋与湖沼,2013,44(2):525-531.

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*大竹蛏(Solen grandis)不同地理群体遗传多样性的AFLP分析*
张滔¹, 刘相全², 孙振兴³, 乔洪金², 孙国华², 刘丽娟², 宋向军²
1.山东省海洋水产研究所,鲁东大学生命科学学院;2.山东省海洋水产研究所,山东省海洋生态修复重点实验室;3.鲁东大学生命科学学院

摘要:

应用AFLP分子标记技术对我国沿海大竹蛏(Solen grandis)群体的遗传多样性进行了分析, 利用筛选出的7对AFLP引物, 对丹东、烟台、莱州、日照和南通5个地理群体的141只大竹蛏个体进行了扩增, 共得到了403个位点, 片段大小在100—700bp之间。其中丹东、烟台、莱州、南通4 个群体的大竹蛏遗传多样性水平接近, 日照群体的多态位点比例高于其它4 个群体, 结合Shannon’s信息指数(I)和Nei’s基因多样性指数(H)看, 日照群体的遗传多样性水平最高, 而南通群体的遗传多样性水平最低。AMOVA分析得到群体间遗传分化系数Φst= 0.2250 (P<0.001), 群体间的变异百分率为22.5%, 群体内为77.5%, 说明群体的遗传变异主要来源于群体内个体间的遗传差异, 但群体间也存在一定程度的基因渗透。

关键词: 大竹蛏 AFLP 遗传多样性

DOI：10.11693/hyhz201302041041

分类号:

基金项目:山东省农业良种工程课题“优质高产抗逆贝类良种选育”(2009—2013)资助; 水生动物营养与饲料泰山学者岗位经费资助, TS200651036号。

附件

GENETIC DIVERSITY OF FIVE GEOGRAPHICAL POPULATIONS IN SOLEN GRANDISBY AFLP MARKERS

ZHANG Tao¹, LIU Xiang-Quan², SUN Zhen-Xing³, QIAO Hong-Jin², SUN Guo-Hua², LIU Li-Juan², SONG Xiang-Jun²

1.Shandong Marine Fisheries Research Institute,College of Life Science, Ludong University;2.Shandong Marine Fisheries Research Institute,Shandong Provincial Key Laboratory of Restoration for Marine Ecology;3.College of Life Science, Ludong University

Abstract:

The AFLP (Amplified fragment length polymorphism) technology was used to estimate the genetic diversity of Solen grandis from several coastal sites of China. 141 natural individuals from 5 geographical populations of S. grandis located at Dandong (DD), Yantai (YT), Laizhou (LZ), Rizhao (RZ), and Nantong (NT) were analyzed using 7 pairs of AFLP primers. A total of 403 reproducible loci were generated, most of which varied from 100bp to 700bp. There was little different in the genetic diversity of DD, YT, LZ and NT, whereas the ratio of polymorphic loci of RZ was relatively higher than the other four populations. Combined with the Shannon’s index and Nei's genetic diversity index, RZ had the highest genetic diversity and NT had the lowest. AMOVA analysis showed that the genetic differentiation coefficient of S. grandis populations (Φst) was 0.2250 (P<0.001) and there was 77.5% genetic differentiation within the populations and 22.5% among the populations. These results indicated the genetic diversity detected was mainly caused by individual differences within a population, but there was somewhat gene flow between the populations.

Key words: Solen grandis AFLP genetic diversity